Hi, I am new to using Bioconductor. I was trying to load my CEL files using ReadAffy(widget=TRUE) and it gave me this message: Error in fileBrowser(textToShow = "Choose CEL files", testFun = hasSuffix("[cC][eE][lL]")) : couldn't find function "tktoplevel" Error in end() : couldn't find function "tkgrab.release" Can you help? Thanks, Tina Naik

Tina, Make sure that you have downloaded all of the "affy" and "tk" files, as well as biobase. There are a lot of dependencies in the software. --Naomi At 11:20 AM 6/10/2004 -0500, tn2@cec.wustl.edu wrote: >Hi, > >I am new to using Bioconductor. I was trying to load my CEL files using > >ReadAffy(widget=TRUE) > >and it gave me this message: > >Error in fileBrowser(textToShow = "Choose CEL files", testFun = >hasSuffix("[cC][eE][lL]")) : > couldn't find function "tktoplevel" >Error in end() : couldn't find function "tkgrab.release" > >Can you help? > >Thanks, >Tina Naik > >_______________________________________________ >Bioconductor mailing list >Bioconductor@stat.math.ethz.ch >https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor Naomi S. Altman 814-865-3791 (voice) Associate Professor Bioinformatics Consulting Center Dept. of Statistics 814-863-7114 (fax) Penn State University 814-865-1348 (Statistics) University Park, PA 16802-2111

Hi Tina, Using the widget=TRUE option requires the tcltk package to be installed and functioning in R. What happens if (after loading a new R session) you type: library(tcltk) OR capabilities("tcltk") ? OR library(tkWidgets) If these all work OK (without errors), then maybe an affy function just forgot to make sure the tcltk package is loaded, in which case just make sure you have typed "library(tkWidgets)" (which loads "tcltk") before you run "affy". But I suspect you may have a problem with your tcltk installation. Please let us know which operating system you are using (Windows, Mac OSX, Linux, ...) and which version of R you are running: Type "version" at the R prompt. And which version of the "affy" package and the "tkWidgets" package you are running, e.g. with packageDescription("affy") packageDescription("tkWidgets") If none of these things help to solve the problem, then perhaps Jianhua Zhang (author of tkWidgets) can help. It is possible that you installed R without tcltk (e.g. using the "mini" installer for Windows). Or it's possible in Linux/Unix that your R installation method may not have found the Tcl/Tk libraries in the correct directory, e.g. /usr/lib/ or /usr/share/ or wherever. Regards, James