problem with pd.huex.1.0.st.v2 and oligo::getProbeInfo
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@mikhail-pachkov-2806
Last seen 9.7 years ago
Dear All, I have got a strange problem. I try to get probe information (particularly I am interested in fsetid, fid, atom) with getProbeInfo function. Here is example: library(oligo); rawdata=read.celfiles("GSM529785.CEL.gz"); probeInfo <- getProbeInfo(rawdata, c('fid', 'fsetid', 'atom', 'transcript_cluster_id','exon_id')); However it looks like instead of fsetid function returns transcript_cluster_id: >probeInfo[1,] fid man_fsetid fsetid atom transcript_cluster_id exon 1 15 3029030 3029030 5132751 3029030 444853 If I check directly underlying database pd.huex.1.0.st.v2/extdata/pd.huex.1.0.st.v2.sqlite I can see that function returned transcript_cluster_id instead of fsetid. >select * from pmfeature where atom=5132751; fid|fsetid | atom | x | y 15|3029044|5132751|14|0 sqlite> select * from featureSet where fsetid=3029044; fsetid|strand|start|stop|transcript_cluster_id|exon_id|crosshyb_type|l evel|chrom|type 3029044|0|142991717|142991843|3029030|444853|1|1|9|1 Do I use wrong arguments for getProbeInfo? Is it bug? Thank you in advance! Best regards, Mikhail Pachkov
probe probe • 1.2k views
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@benilton-carvalho-1375
Last seen 4.1 years ago
Brazil/Campinas/UNICAMP
getProbeInfo() has an argument called 'target', which (for Exon/Gene ST arrays) is set by default to 'core'. Looks like you want to add this argument and set it to 'probeset'. probeInfo <- getProbeInfo(rawdata, c('fid', 'fsetid', 'atom', 'transcript_cluster_id','exon_id'), target='probeset') Best wishes, b 2013/2/14 Mikhail Pachkov <pachkov at="" gmail.com="">: > Dear All, > > I have got a strange problem. I try to get probe information > (particularly I am interested in fsetid, fid, atom) with getProbeInfo > function. Here is example: > > library(oligo); > rawdata=read.celfiles("GSM529785.CEL.gz"); > probeInfo <- getProbeInfo(rawdata, c('fid', 'fsetid', 'atom', > 'transcript_cluster_id','exon_id')); > > However it looks like instead of fsetid function returns transcript_cluster_id: > >>probeInfo[1,] > fid man_fsetid fsetid atom transcript_cluster_id exon > 1 15 3029030 3029030 5132751 3029030 444853 > > If I check directly underlying database > pd.huex.1.0.st.v2/extdata/pd.huex.1.0.st.v2.sqlite I can see that > function returned transcript_cluster_id instead of fsetid. > >>select * from pmfeature where atom=5132751; > fid|fsetid | atom | x | y > 15|3029044|5132751|14|0 > > sqlite> select * from featureSet where fsetid=3029044; > fsetid|strand|start|stop|transcript_cluster_id|exon_id|crosshyb_type |level|chrom|type > 3029044|0|142991717|142991843|3029030|444853|1|1|9|1 > > Do I use wrong arguments for getProbeInfo? Is it bug? > > Thank you in advance! > > Best regards, > > Mikhail Pachkov > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor
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Thanks! It worked! Best regards, Mikhail On Thu, Feb 14, 2013 at 3:35 PM, Benilton Carvalho <beniltoncarvalho at="" gmail.com=""> wrote: > getProbeInfo() has an argument called 'target', which (for Exon/Gene > ST arrays) is set by default to 'core'. > > Looks like you want to add this argument and set it to 'probeset'. > > probeInfo <- getProbeInfo(rawdata, c('fid', 'fsetid', 'atom', > 'transcript_cluster_id','exon_id'), target='probeset') > > Best wishes, b > > 2013/2/14 Mikhail Pachkov <pachkov at="" gmail.com="">: >> Dear All, >> >> I have got a strange problem. I try to get probe information >> (particularly I am interested in fsetid, fid, atom) with getProbeInfo >> function. Here is example: >> >> library(oligo); >> rawdata=read.celfiles("GSM529785.CEL.gz"); >> probeInfo <- getProbeInfo(rawdata, c('fid', 'fsetid', 'atom', >> 'transcript_cluster_id','exon_id')); >> >> However it looks like instead of fsetid function returns transcript_cluster_id: >> >>>probeInfo[1,] >> fid man_fsetid fsetid atom transcript_cluster_id exon >> 1 15 3029030 3029030 5132751 3029030 444853 >> >> If I check directly underlying database >> pd.huex.1.0.st.v2/extdata/pd.huex.1.0.st.v2.sqlite I can see that >> function returned transcript_cluster_id instead of fsetid. >> >>>select * from pmfeature where atom=5132751; >> fid|fsetid | atom | x | y >> 15|3029044|5132751|14|0 >> >> sqlite> select * from featureSet where fsetid=3029044; >> fsetid|strand|start|stop|transcript_cluster_id|exon_id|crosshyb_typ e|level|chrom|type >> 3029044|0|142991717|142991843|3029030|444853|1|1|9|1 >> >> Do I use wrong arguments for getProbeInfo? Is it bug? >> >> Thank you in advance! >> >> Best regards, >> >> Mikhail Pachkov >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at r-project.org >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor
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