ChIPpeakAnno "examplePattern.fa" file
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@jose-luis-lavin-5797
Last seen 9.6 years ago
When looking for motif occurrence in the peaks, the "examplePattern.fa" file you supply with the module: -Is a standard example pattern file you can use for any set under analysis or is it a custom one you include in the package referred to a particular set of motifs? -May you give a hint on how to create such kind of file myself? Thanks in advance JL [[alternative HTML version deleted]]
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Julie Zhu ★ 4.3k
@julie-zhu-3596
Last seen 5 months ago
United States
Jose, Thanks for the inquiry! I will add the following to the documentation. The examplePattern.fa, in fasta format, is included in the package as an example pattern file for running function summarizePatternInPeaks. To use this function, you will need to create your own custom pattern file with the pattern(s) you are interested in searching in the peaks. The pattern file is in fasta file format. The base in the pattern consists of IUPAC designated nucleotide symbols such as A, C, G, T, N, V, H, D.... The bottom table on http://pgfe.umassmed.edu/ZFPsearch.html lists the abbreviation codes for degenerate bases. Best regards, Julie On 2/27/13 10:54 AM, "Jos? Luis Lav?n" <joluito at="" gmail.com=""> wrote: > When looking for motif occurrence in the peaks, the "examplePattern.fa" file > you supply with the module: > > -Is a standard example pattern file you can use for any set under analysis or > is it a custom one you include in the package referred to a particular set of > motifs? > > -May you give a hint on how to create such kind of file myself? > > Thanks in advance > > JL > > > >
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Thank you Julie, I misunderstood the functionality and thought I was a "de novo" motif finder... Any insight on how to predict motifs from ChIPseq experiments? Any R module that does it and you can recommend? May you think of implementing this function to your awesome package? ;) JL 2013/2/27 Zhu, Lihua (Julie) <julie.zhu@umassmed.edu> > Jose, > > Thanks for the inquiry! I will add the following to the documentation. > > The examplePattern.fa, in fasta format, is included in the package as an > example pattern file for running function summarizePatternInPeaks. To use > this function, you will need to create your own custom pattern file with > the > pattern(s) you are interested in searching in the peaks. > > The pattern file is in fasta file format. The base in the pattern consists > of IUPAC designated nucleotide symbols such as A, C, G, T, N, V, H, D.... > > The bottom table on http://pgfe.umassmed.edu/ZFPsearch.html lists the > abbreviation codes for degenerate bases. > > Best regards, > > Julie > > > On 2/27/13 10:54 AM, "José Luis Lavín" <joluito@gmail.com> wrote: > > > When looking for motif occurrence in the peaks, the "examplePattern.fa" > file > > you supply with the module: > > > > -Is a standard example pattern file you can use for any set under > analysis or > > is it a custom one you include in the package referred to a particular > set of > > motifs? > > > > -May you give a hint on how to create such kind of file myself? > > > > Thanks in advance > > > > JL > > > > > > > > > > -- -- José Luis Lavín Trueba, Ph.D Genome Analysis Platform CIC bioGUNE Parque Tecnológico de Bizkaia Building 502, Floor 0 48160 Derio-Spain Tel.: +34 946 572 524 Fax: +34 946 568 732 [[alternative HTML version deleted]]
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Jose, Thanks for your kind word! You might want to check out rGADEM package for de nova motif discovery. Best regards, Julie On 2/27/13 11:29 AM, "Jos? Luis Lav?n" <joluito at="" gmail.com=""> wrote: > Thank you Julie, > > I misunderstood the functionality and thought I was a "de novo" motif > finder... > Any insight on how to predict motifs from ChIPseq experiments? Any R module > that does it and you can recommend? May you think of implementing this > function to your awesome package? > ;) > > JL > > 2013/2/27 Zhu, Lihua (Julie) <julie.zhu at="" umassmed.edu=""> >> Jose, >> >> Thanks for the inquiry! I will add the following to the documentation. >> >> The examplePattern.fa, in fasta format, is included in the package as an >> example pattern file for running function summarizePatternInPeaks. To use >> this function, you will need to create your own custom pattern file with the >> pattern(s) you are interested in searching in the peaks. >> >> The pattern file is in fasta file format. The base in the pattern consists >> of IUPAC designated nucleotide symbols such as A, C, G, T, N, V, H, D.... >> >> The bottom table on http://pgfe.umassmed.edu/ZFPsearch.html lists the >> abbreviation codes for degenerate bases. >> >> Best regards, >> >> Julie >> >> >> On 2/27/13 10:54 AM, "Jos? Luis Lav?n" <joluito at="" gmail.com=""> wrote: >> >>> When looking for motif occurrence in the peaks, the "examplePattern.fa" file >>> you supply with the module: >>> >>> -Is a standard example pattern file you can use for any set under analysis >>> or >>> is it a custom one you include in the package referred to a particular set >>> of >>> motifs? >>> >>> -May you give a hint on how to create such kind of file myself? >>> >>> Thanks in advance >>> >>> JL >>> >>> >>> >>> >> > >
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