Hi to all, I wanted to read in the files in the same order of the target file but I can't read the files names from the target file because the function wants a 2 columns matrix... so that I created the matrix: > oxyfiles<-cbind(Oxytarget$FileNameCy3,Oxytarget$FileNameCy5) Now I have a matrix with the files in the same order of the target files but when I try to read in the data I get this message: > RGo<-read.imagene(oxyfiles,columns=list(f="Median Signal",b="Background Median"),names=Oxytarget$Name,path="C:/Program Files/R/rw1081/bin/oxyR") Read header information Read C:/Program Files/R/rw1081/bin/oxyR/2-4-04_SC19.57_Cy3_g58.txt Error in "[.data.frame"(obj, , columns$f) : undefined columns selected Please can somebody tell me what I'm doing wrong? I find inconvenient use the command dir(pattern=*.txt) to create the file with the arrays because it stores them randomly... Thank you very much in advance. best regards. Emanuele

Hi to all, I wanted to read in the files in the same order of the target file but I can't read the files names from the target file because the function wants a 2 columns matrix... so that I created the matrix: > oxyfiles<-cbind(Oxytarget$FileNameCy3,Oxytarget$FileNameCy5) Now I have a matrix with the files in the same order of the target files but when I try to read in the data I get this message: > RGo<-read.imagene(oxyfiles,columns=list(f="Median Signal",b="Background Median"),names=Oxytarget$Name,path="C:/Program Files/R/rw1081/bin/oxyR") Read header information Read C:/Program Files/R/rw1081/bin/oxyR/2-4-04_SC19.57_Cy3_g58.txt Error in "[.data.frame"(obj, , columns$f) : undefined columns selected Please can somebody tell me what I'm doing wrong? I find inconvenient use the command dir(pattern=*.txt) to create the file with the arrays because it stores them randomly... Thank you very much in advance. best regards. Emanuele