The error message is telling you that you have specified a foreground column name that doesn't exist. Is it possible that you meant "Signal Median" instead of "Median Signal"? ImaGene files do not have a column by the name you gave Gordon At 03:08 AM 1/07/2004, marchiem\@libero\.it wrote: >Hi to all, >I wanted to read in the files in the same order of the target file but I >can't read the files names from the target file because the function wants >a 2 columns matrix... >so that I created the matrix: > > oxyfiles<-cbind(Oxytarget$FileNameCy3,Oxytarget$FileNameCy5) > >Now I have a matrix with the files in the same order of the target files >but when I try to read in the data I get this message: > > > > RGo<-read.imagene(oxyfiles,columns=list(f="Median Signal",b="Background > Median"),names=Oxytarget$Name,path="C:/Program Files/R/rw1081/bin/oxyR") > >Read header information >Read C:/Program Files/R/rw1081/bin/oxyR/2-4-04_SC19.57_Cy3_g58.txt >Error in "[.data.frame"(obj, , columns$f) : > undefined columns selected > >Please can somebody tell me what I'm doing wrong? I find inconvenient use >the command dir(pattern=*.txt) to create the file with the arrays because >it stores them randomly... > >Thank you very much in advance. > >best regards. > >Emanuele