mogene20stcdf
1
0
Entering edit mode
Nikul Soni ▴ 80
@nikul-soni-5917
Last seen 8.2 years ago
Sweden
Hi all, I am a beginner in R and i use R studio to analyse my mouse liver arrays. I am doing my micro array analysis and it misses cdf environment. I check all the database in R and i cant found for the new chip Mogene20 and its database i.e. its .db file. is there any possibility that i can download it from other sources. Regards Nikul -- सर्वेsत्र सुखिन: सन्तु सर्वे सन्तु निरामया: | सर्वे भद्राणि पश्यन्तु मा कश्चित दु:ख माप्नुयात || [[alternative HTML version deleted]]
cdf cdf • 926 views
ADD COMMENT
0
Entering edit mode
@james-w-macdonald-5106
Last seen 2 days ago
United States
Hi Nikul, You won't be able to use the affy package to process these files. Instead you can use either oligo or xps. As for the .db file, those files are supplied by Arthur Li, and apparently he hasn't made them for the 2.0 series. There is apparently a way to add the annotation to xps, but you will have to either look at the help pages or vignettes or wait for Christian Stratowa to chime in. You can also use the AnnotationForge package to create your own .db file, but that is a relatively involved process, as the annotation data from Affy are not that easy to parse. Best, Jim On 5/2/2013 6:55 AM, Nikul Soni wrote: > Hi all, > > I am a beginner in R and i use R studio to analyse my mouse liver arrays. I > am doing my micro array analysis and it misses cdf environment. I check all > the database in R and i cant found for the new chip Mogene20 and its > database i.e. its .db file. is there any possibility that i can download it > from other sources. > > Regards > Nikul > > > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, M.S. Biostatistician University of Washington Environmental and Occupational Health Sciences 4225 Roosevelt Way NE, # 100 Seattle WA 98105-6099
ADD COMMENT
0
Entering edit mode
Dear Nikul, As Jim mentioned, you could use xps. You can find an example how to create the 'scheme' for MoGene 2.0 in ../xps/examples/script4schemes.R. However, first you would need to download the library and annotation files of MoGene 2.0 from the Affymetrix web-site. (Furthermore, please read the README file how to install ROOT first.) Best regards Christian _._._._._._._._._._._._._._._._._._ C.h.r.i.s.t.i.a.n S.t.r.a.t.o.w.a V.i.e.n.n.a A.u.s.t.r.i.a e.m.a.i.l: cstrato at aon.at _._._._._._._._._._._._._._._._._._ On 5/2/13 3:09 PM, James W. MacDonald wrote: > Hi Nikul, > > You won't be able to use the affy package to process these files. > Instead you can use either oligo or xps. As for the .db file, those > files are supplied by Arthur Li, and apparently he hasn't made them for > the 2.0 series. There is apparently a way to add the annotation to xps, > but you will have to either look at the help pages or vignettes or wait > for Christian Stratowa to chime in. > > You can also use the AnnotationForge package to create your own .db > file, but that is a relatively involved process, as the annotation data > from Affy are not that easy to parse. > > Best, > > Jim > > > > On 5/2/2013 6:55 AM, Nikul Soni wrote: >> Hi all, >> >> I am a beginner in R and i use R studio to analyse my mouse liver >> arrays. I >> am doing my micro array analysis and it misses cdf environment. I >> check all >> the database in R and i cant found for the new chip Mogene20 and its >> database i.e. its .db file. is there any possibility that i can >> download it >> from other sources. >> >> Regards >> Nikul >> >> >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at r-project.org >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor >
ADD REPLY

Login before adding your answer.

Traffic: 471 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6