Hi Adrian, I use LimmaGUI and if you make sure you have the latest version there is the custom/other load option. I have found that if you open an example table in excel and copy the right column headings into the Rf, Rb, Gf and Gb labels in the box that comes up I can load data that is from a variety of table types form a variety of spot finding softwares. The typing for the column headings needs to be exact or the load fails. For instance I like to load the median data columns from GenePix and therefore don't use the GenePix file type directly as it uses the mean values. Liz Molteno Building Department of Pathology University of Cambridge Tennis Court Road Cambridge, CB2 1QP United Kingdom Website: http://www.path.cam.ac.uk/~toxo/ Tel 01223 33 33 31(office) or 01223 33 33 29 (lab) ---------------------------------------------------------------------- Message: 1 Date: Thu, 22 Jul 2004 11:57:01 +0200 From: Adrian Peres <adrian.peres@cropdesign.com> Subject: [BioC] limmaGUI and Agilent output files! To: bioconductor@stat.math.ethz.ch Message-ID: <40FF8F6D.10709@cropdesign.com> Content-Type: text/plain; charset=us-ascii; format=flowed Hi everyone Is anybody out there using limmaGUI for microarray analysis? I have Agilent output .txt files and I wonder if they can be imported in limmaGUI. I have tried but it doesn't work! Can anyone give a hint on how to start...? This program is totally new for me and using the "Help" I could not figure out! Thank, Adrian confidentiality notice: The information contained in this e-mail is confidential and...{{dropped}}