XPS package QC data
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b a ▴ 40
@b-a-6096
Last seen 10.2 years ago
Dear colleagues, I use xps package for my HuGene ST 1.0 array and need some help in quality control. I used arrayQualityMetrics package before new version of R was avaliable which doesn't maintain affy package for ST arrays. ArrayQualityMetrics creates good report with "outliers" assays, which are out of borders (for example, cut-offs for NUSE is 1.1), but I've not seen this in xps. Is it possible to receive this data (median and IQR for NUSE or RLE) using xps package? Thank you! Kirill Prusov
affy arrayQualityMetrics xps affy arrayQualityMetrics xps • 1.5k views
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cstrato ★ 3.9k
@cstrato-908
Last seen 6.1 years ago
Austria
Dear Kirill, Please have a look at chapter 5 of vignette 'xps.pdf', especially chapter 5.4 describing class QualTreeSet. See also the corresponding help files, e.g. '?plotNUSE'. '?plotRLE', '?plotMAD', '?plotCOI' etc. Furthermore, please look at function 'xpsQAReport()' which will create a quality report 'QAReport.pdf'. Best regards Christian _._._._._._._._._._._._._._._._._._ C.h.r.i.s.t.i.a.n S.t.r.a.t.o.w.a V.i.e.n.n.a A.u.s.t.r.i.a e.m.a.i.l: cstrato at aon.at _._._._._._._._._._._._._._._._._._ On 8/16/13 1:58 PM, b a wrote: > Dear colleagues, > > I use xps package for my HuGene ST 1.0 array and need some help in quality control. I used arrayQualityMetrics package before new version of R was avaliable which doesn't maintain affy package for ST arrays. ArrayQualityMetrics creates good report with "outliers" assays, which are out of borders (for example, cut-offs for NUSE is 1.1), but I've not seen this in xps. > > Is it possible to receive this data (median and IQR for NUSE or RLE) using xps package? > > Thank you! > > Kirill Prusov > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor >
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Dear Christian, Thank you for this answer. I'm trying to use this package for automatic quality control. In arrayQualityMetrics report I can see table with detected outliers (such as here: http://www- huber.embl.de/arrayQualityMetrics/Report_for_nMLL_with_factors/) In the xps package I cannot make my report in pdf because I have some errors, only Rwd file and all images. Does pdf file contain more information than I see into the QC stats folder? Thank you! And one more question. I have an error when I launch xps package at Windows 7. The error is "The procedure entry point _ZN18G_FastAllocString6FormatEPKcz could not be located in the dynamic link library libCint.dll" > sessionInfo() R version 3.0.1 (2013-05-16) Platform: i386-w64-mingw32/i386 (32-bit) locale: [1] LC_COLLATE=Russian_Russia.1251 LC_CTYPE=Russian_Russia.1251 LC_MONETARY=Russian_Russia.1251 LC_NUMERIC=C LC_TIME=Russian_Russia.1251 attached base packages: [1] stats graphics grDevices utils datasets methods base other attached packages: [1] BiocInstaller_1.10.3 loaded via a namespace (and not attached): [1] tools_3.0.1 16.08.2013, 21:51, "cstrato" <cstrato at="" aon.at="">: > ?Dear Kirill, > > ?Please have a look at chapter 5 of vignette 'xps.pdf', especially > ?chapter 5.4 describing class QualTreeSet. See also the corresponding > ?help files, e.g. '?plotNUSE'. '?plotRLE', '?plotMAD', '?plotCOI' etc. > > ?Furthermore, please look at function 'xpsQAReport()' which will create a > ?quality report 'QAReport.pdf'. > > ?Best regards > ?Christian > ?_._._._._._._._._._._._._._._._._._ > ?C.h.r.i.s.t.i.a.n ??S.t.r.a.t.o.w.a > ?V.i.e.n.n.a ??????????A.u.s.t.r.i.a > ?e.m.a.i.l: ???????cstrato at aon.at > ?_._._._._._._._._._._._._._._._._._ > > ?On 8/16/13 1:58 PM, b a wrote: >> ??Dear colleagues, >> >> ??I use xps package for my HuGene ST 1.0 array and need some help in quality control. I used arrayQualityMetrics package before new version of R was avaliable which doesn't maintain affy package for ST arrays. ArrayQualityMetrics creates good report with "outliers" assays, which are out of borders (for example, cut-offs for NUSE is 1.1), but I've not seen this in xps. >> >> ??Is it possible to receive this data (median and IQR for NUSE or RLE) using xps package? >> >> ??Thank you! >> >> ??Kirill Prusov >> >> ??_______________________________________________ >> ??Bioconductor mailing list >> ??Bioconductor at r-project.org >> ??https://stat.ethz.ch/mailman/listinfo/bioconductor >> ??Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor
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Dear Kirill, To answer your first question: No, the pdf file does not contain more information than shown in the QC stats folder, it is only a conversion of the QAReport.Rnw file. BTW, I have just tried to run function 'xpsQAReport()' and get the following error: Loading required package: tools Error in .get_package_metadata(pkgdir) : Files 'DESCRIPTION' and 'DESCRIPTION.in' are missing. Is this the error that you get? It seems that function buildVignettes() of the 'tools' package must have changed since this error did not appear in earlier versions of R!! I will ask at the R-devel mailing list what the error might be. BTW, if you want to get also numeric QC values please have a look at function treeInfo() and the examples shown in the help '?treeInfo'. Regarding your second question: - What is the name of the ROOT binary that you have installed on your current machine? (Is this WinXP?) - What is the name of the ROOT binary that you did install on Windows 7? Best regards, Christian On 8/28/13 10:46 AM, b a wrote: > Dear Christian, > > Thank you for this answer. I'm trying to use this package for automatic quality control. In arrayQualityMetrics report I can see table with detected outliers (such as here: http://www- huber.embl.de/arrayQualityMetrics/Report_for_nMLL_with_factors/) In the xps package I cannot make my report in pdf because I have some errors, only Rwd file and all images. Does pdf file contain more information than I see into the QC stats folder? > > Thank you! > > And one more question. I have an error when I launch xps package at Windows 7. The error is "The procedure entry point _ZN18G_FastAllocString6FormatEPKcz could not be located in the dynamic link library libCint.dll" > > >> sessionInfo() > R version 3.0.1 (2013-05-16) > Platform: i386-w64-mingw32/i386 (32-bit) > > locale: > [1] LC_COLLATE=Russian_Russia.1251 LC_CTYPE=Russian_Russia.1251 LC_MONETARY=Russian_Russia.1251 LC_NUMERIC=C LC_TIME=Russian_Russia.1251 > > attached base packages: > [1] stats graphics grDevices utils datasets methods base > > other attached packages: > [1] BiocInstaller_1.10.3 > > loaded via a namespace (and not attached): > [1] tools_3.0.1 > > > 16.08.2013, 21:51, "cstrato" <cstrato at="" aon.at="">: > >> Dear Kirill, >> >> Please have a look at chapter 5 of vignette 'xps.pdf', especially >> chapter 5.4 describing class QualTreeSet. See also the corresponding >> help files, e.g. '?plotNUSE'. '?plotRLE', '?plotMAD', '?plotCOI' etc. >> >> Furthermore, please look at function 'xpsQAReport()' which will create a >> quality report 'QAReport.pdf'. >> >> Best regards >> Christian >> _._._._._._._._._._._._._._._._._._ >> C.h.r.i.s.t.i.a.n S.t.r.a.t.o.w.a >> V.i.e.n.n.a A.u.s.t.r.i.a >> e.m.a.i.l: cstrato at aon.at >> _._._._._._._._._._._._._._._._._._ >> >> On 8/16/13 1:58 PM, b a wrote: >>> Dear colleagues, >>> >>> I use xps package for my HuGene ST 1.0 array and need some help in quality control. I used arrayQualityMetrics package before new version of R was avaliable which doesn't maintain affy package for ST arrays. ArrayQualityMetrics creates good report with "outliers" assays, which are out of borders (for example, cut-offs for NUSE is 1.1), but I've not seen this in xps. >>> >>> Is it possible to receive this data (median and IQR for NUSE or RLE) using xps package? >>> >>> Thank you! >>> >>> Kirill Prusov >>> >>> _______________________________________________ >>> Bioconductor mailing list >>> Bioconductor at r-project.org >>> https://stat.ethz.ch/mailman/listinfo/bioconductor >>> Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor >
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