Hello Heidi, I'm a real beginner in R and would like to analyze my miRNAs TLDA data with your R-package. I have 60 patients with 384*2 wells , I 'd like to use the following features in order to make nice normalization with your software. Well position patient type of cancer miRNA Ct A1 001 CRC hsa-miR-1 27.33 target A2 001 CRC has-miR-3 23.2 Endogenous Control ... In the same folder I put a : - 1 files.txt as mentioned in the description of the package and put inside the description of the samples to analyze (i.e the description of the patient and the corresponding .txt) - Each .txt of each samples which were described in the files.txt. I tried: library("HTqPCR") head(read.delim(file.path("D:/1-prestizia/laboratoire/protocole-stat- han dbook/statistique/R/HTqPCR/test-gui/test-gui", "files.txt"))) files <- read.delim(file.path("D:/1-prestizia/laboratoire/protocole-stat- handbook /statistique/R/HTqPCR/test-gui/test-gui", "files.txt")) and my problem is below! The readCtData function !!! impossible to open the sample1.txt! """"No such file or directory"""" raw <- readCtData (files = files$File, path = path)) #show(raw) Help J Thank you in advance for your help! Guillaume [[alternative HTML version deleted]]