Hi all, my name is Luca Peruzza, I am a student working at the University of Trieste. I approached R software because I have to calculate Gene Expression and I found this R library ("ddCt") that is exactly what i need because I need to calculate the Gene Expression, the propagation of the error, and I want to perform statistical analysis with my data. In the laboratory we have a Biorad RT-PCR. I can export the file generated from the Biorad software onto Exel. I'm new to R, but I tried to use the package ddCt with my chief (who knows R) and we read all the documentation about the package, but we don't understand how to use it. In particular we can't understand the following: - how has to be the file that you load? - how can the package understand which gene is the reference, which one is the target, .... - how can you perform a t-test using the data? I can provide an example of the output of the qPCR, if needed. Can someone be so kind to explain how to use it? Would be the best if you could provide us a script with the commands and explanations of them! Thanking you in advance for the time you spent with me, I want to wish you a pleasant journey. -- output of sessionInfo(): load.path = "." -- Sent via the guest posting facility at bioconductor.org.