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Guest User ★ 12k@guest-user-4897
Last seen 7.1 years ago
Dear Bioconductor users, I would like to plot heatmap with metagenomics dataset, I have 48 mouse samples that were distributed into 8 groups. I copied my dataset as the same format to metagenomeSeq directory for importing the dataset. Everything seems fine till I tried to plot heatmap. I copied the scripts from the tutorial. > data(mouseData) > trials = pData(mouseData)$diet > heatmapColColors = brewer.pal(12, "Set3")[as.integer(factor(trials))] > heatmapCols = colorRampPalette(brewer.pal(9, "RdBu"))(50) > # plotMRheatmap > plotMRheatmap(obj = mouseData, n = 200, cexRow = 0.4, cexCol = 0.4, trace = "none", col = heatmapCols, ColSideColors = heatmapColColors) When I looked at the heatmap, it is not my dataset, it is still from the example dataset. When I checked on the scripts again, I didn't see any script to help build mouseData with the new dataset. COuld you instruct how I load my dataset into mouseData? or any other solutions? Thanks a lot, I do appreciate it. -- output of sessionInfo(): > sessionInfo() R version 3.0.2 (2013-09-25) Platform: x86_64-apple-darwin10.8.0 (64-bit) locale:  en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8 attached base packages:  parallel stats graphics grDevices utils datasets methods base other attached packages:  metagenomeSeq_1.4.0 gplots_2.12.1 RColorBrewer_1.0-5 matrixStats_0.8.14 limma_3.18.13 Biobase_2.22.0 BiocGenerics_0.8.0  BiocInstaller_1.12.0 loaded via a namespace (and not attached):  bitops_1.0-6 caTools_1.16 gdata_2.13.2 gtools_3.3.1 KernSmooth_2.23-10 R.methodsS3_1.6.1 tools_3.0.2 > -- Sent via the guest posting facility at bioconductor.org.