Dear Maria, Are you simply asking how to join sequencing libraries together? If you have two matrices of counts (with the same number of rows representing the same genes or transcripts in the same order), then you can join them together using cbind: counts <- cbind(counts1, counts2) Then make your DGEList as usual: d <- DGEList(counts=counts) etc. Best wishes Gordon > Date: Mon, 31 Mar 2014 15:28:22 +0200 > From: Mar?a Jes?s Garc?a <mj.garcia.pereira at="" gmail.com=""> > To: bioconductor at r-project.org > Subject: [BioC] Join expression values of replicas in edgeR > > I'm working with RNAseq data and I would like to join the expression values > for the replicas of the different treatments in edgeR. I would like to plot > some figures with the combined information of my replicas, as I have found > some confusing results when they are plotted invidually. Is there a way to > do this in edgeR or maybe I could compute the mean expression value of > samples over treatments and pass this matrix to edgeR? > > Thank you, > > -- > Mar?a Jes?s Garc?a Pereira ______________________________________________________________________ The information in this email is confidential and intend...{{dropped:5}}