reading qRT-PCR data
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Guest User ★ 12k
Last seen 8.4 years ago
Hello, I was wondering how I can read in qRT-PCR data from our system output for use with the ddCt package. We have an ABI Quant Studio 6 machine and I can export the data in 7900 format. However, at this point, I'm not too clear what happens next. After searching, I came across some packages such as HTqPCR or ReadqPCR that can import data in SDS format (our machine exports SDS 2.3), but still, sometimes the formatting is not exact and. Does anyone have any experience in using ABI machines and processing the data through Bioconductor? Thanks. -- output of sessionInfo(): R version 3.0.2 (2013-09-25) Platform: x86_64-apple-darwin10.8.0 (64-bit) locale: [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8 attached base packages: [1] stats graphics grDevices utils datasets methods base loaded via a namespace (and not attached): [1] colorspace_1.2-4 dichromat_2.0-0 digest_0.6.4 ggplot2_0.9.3.1 grid_3.0.2 gtable_0.1.2 [7] labeling_0.2 MASS_7.3-29 munsell_0.4.2 plyr_1.8 proto_0.3-10 RColorBrewer_1.0-5 [13] reshape2_1.2.2 scales_0.2.3 stringr_0.6.2 -- Sent via the guest posting facility at
ddCt HTqPCR ReadqPCR ddCt HTqPCR ReadqPCR • 1.7k views

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