If you type 'listgtMethods()', it will give you a list of registered methods Gating Functions: === quantileGate === rangeGate === flowClust.2d === mindensity ... If your method is not in the list, then you should first register it by 'registerPlugins', e.g. registerPlugins(fun = .flowDensity, methodName = "flowDensity") Mike On 08/12/2014 06:33 AM, DELANNOY Claire wrote: > > Dear Mr Jiang and Mr Finak, > > I try to do an automated gating on mass cytometry data. I wanted to > use openCyto , it worked to define my Singlets population with flowClust. > > Then I defined my Live singlets with flowDensity and I got an error : > > /Gating for 'liveSinglets'/ > > /Erreur dans (function (fs, pp_res, gFunc, popAlias, channels, ...) : / > > / Can't gate using unregistered method .flowDensity/ > > I read your posts on Github and especially the issue #62 . I wrote my > gating template in the same way as you (I attached the csv file and > the R script if needed) > > Do I need to do something to ?register? flowDensity so that openCyto > can use it? > > Thanks in advance! > > Claire > > ** > > *Claire Delannoy* > > Technology Research Department ? Bioinformatics Engineer > > Phone: +33 (0) 4 69 85 16 96 > > cid:image002.png at 01CEF5D8.6044DD70 <http: www.bioaster.com=""/> > > 321 Avenue Jean Jaur?s | B?timent Domilyon |69 007 LYON France > > Website : http://www.bioaster.com <http: www.bioaster.com=""/> >