Hi, Quick question this time. Basically these dyes have higher intensity, less quenching and correlate more between dye pairs. The ULYSIS kit uses chemical labelling which eliminates sequence effects. http://www.probes.com/servlets/directory?id1=6&id2=48&id3=319 http://www.probes.com/media/publications/394.pdf As far as I know Limma accounts for dye bias during normalisation. If there is less/no bias will there be artifacts introduced? Or other points I need to be aware of? Thanks, Matt