Hi
Please accept my apologies if this is obvious, it's been some time since I have worked with microarray data.
I have some Affymetrix CEL files that use the HuGene 2.0 ST design (http://www.bioconductor.org/packages/release/data/annotation/html/pd.hugene.2.0.st.html).
Running R 3.1.1, I tried reading these using the Affy package, but Affy refused and recommended I use the oligo or xps package. But neither oligo nor xps install in R 3.1.1 so I downgraded to R 2.15.2.
Now I can install oligo, but it asks for the annotation package - pd.hugene.2.0.st - which is only available for R >= 3.1.0.
My question is - how do I work with HuGene 2.0 ST arrays in Bioconductor? I just want to extract RMA values for input into limma.
Many thanks
Mick