Reading Affymetrix data from pd.hugene.2.0.st using oligo/affy
3
0
Entering edit mode
WATSON Mick ▴ 50
@watson-mick-5575
Last seen 9.9 years ago
United Kingdom

Hi

Please accept my apologies if this is obvious, it's been some time since I have worked with microarray data.

I have some Affymetrix CEL files that use the HuGene 2.0 ST design (http://www.bioconductor.org/packages/release/data/annotation/html/pd.hugene.2.0.st.html).

Running R 3.1.1, I tried reading these using the Affy package, but Affy refused and recommended I use the oligo or xps package.  But neither oligo nor xps install in R 3.1.1 so I downgraded to R 2.15.2.

Now I can install oligo, but it asks for the annotation package - pd.hugene.2.0.st - which is only available for R >= 3.1.0.

My question is - how do I work with HuGene 2.0 ST arrays in Bioconductor?  I just want to extract RMA values for input into limma.

Many thanks

Mick

affy oligo • 2.3k views
ADD COMMENT
0
Entering edit mode
@benilton-carvalho-1375
Last seen 4.7 years ago
Brazil/Campinas/UNICAMP
Can you please describe what error you get when installing oligo on R-3.1.1? I have it running just fine on R 3.1.1 (on Mac OS X) and on R 3.1.2 (on Ubuntu). On Mon Jan 19 2015 at 3:15:43 PM WATSON Mick [bioc] < noreply@bioconductor.org> wrote: > Activity on a post you are following on support.bioconductor.org > > User WATSON Mick <https: support.bioconductor.org="" u="" 5575=""/> wrote Question: > Reading Affymetrix data from pd.hugene.2.0.st using oligo/affy > <https: support.bioconductor.org="" p="" 64214=""/>: > > Hi > > Please accept my apologies if this is obvious, it's been some time since I > have worked with microarray data. > > I have some Affymetrix CEL files that use the HuGene 2.0 ST design ( > http://www.bioconductor.org/packages/release/data/annotation/html/pd.hugene.2.0.st.html) > . > > Running R 3.1.1, I tried reading these using the Affy package, but Affy > refused and recommended I use the oligo or xps package. But neither oligo > nor xps install in R 3.1.1 so I downgraded to R 2.15.2. > > Now I can install oligo, but it asks for the annotation package - > pd.hugene.2.0.st - which is only available for R >= 3.1.0. > > My question is - how do I work with HuGene 2.0 ST arrays in Bioconductor? > I just want to extract RMA values for input into limma. > > Many thanks > > Mick > ------------------------------ > > You may reply via email or visit Reading Affymetrix data from pd.hugene.2.0.st using oligo/affy >
ADD COMMENT
0
Entering edit mode
WATSON Mick ▴ 50
@watson-mick-5575
Last seen 9.9 years ago
United Kingdom
Hi Thanks for the reply. Using biocLite.R, the function said that oligo was not available for R 3.1.1. Do I need to grab the tar.gz instead? Cheers Mick
ADD COMMENT
0
Entering edit mode
It's hard to say without further details. If you send the (full) results of the following commands, I'd be able to help you out. Start a brand-new R session and type: sessionInfo() source('http://www.bioconductor.org/biocLite.R') biocLite('oligo') Thank you On Mon Jan 19 2015 at 6:40:26 PM WATSON Mick [bioc] < noreply@bioconductor.org> wrote: > Activity on a post you are following on support.bioconductor.org > > User WATSON Mick <https: support.bioconductor.org="" u="" 5575=""/> wrote Answer: > Reading Affymetrix data from pd.hugene.2.0.st using oligo/affy > <https: support.bioconductor.org="" p="" 64214="" #64218="">: > > Hi Thanks for the reply. Using biocLite.R, the function said that oligo > was not available for R 3.1.1. Do I need to grab the tar.gz instead? Cheers > Mick > ------------------------------ > > You may reply via email or visit > A: Reading Affymetrix data from pd.hugene.2.0.st using oligo/affy >
ADD REPLY
0
Entering edit mode
Biologist ▴ 120
@biologist-9801
Last seen 4.7 years ago

Hi,

I have some Affymetrix CEL files that use the HuGene 2.0 ST design.

Can I use oligo package for RNA degradation plot?

Thanks

ADD COMMENT

Login before adding your answer.

Traffic: 979 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6