Reading Affymetrix data from pd.hugene.2.0.st using oligo/affy
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WATSON Mick ▴ 50
@watson-mick-5575
Last seen 9.9 years ago
United Kingdom

Hi

Please accept my apologies if this is obvious, it's been some time since I have worked with microarray data.

I have some Affymetrix CEL files that use the HuGene 2.0 ST design (http://www.bioconductor.org/packages/release/data/annotation/html/pd.hugene.2.0.st.html).

Running R 3.1.1, I tried reading these using the Affy package, but Affy refused and recommended I use the oligo or xps package.  But neither oligo nor xps install in R 3.1.1 so I downgraded to R 2.15.2.

Now I can install oligo, but it asks for the annotation package - pd.hugene.2.0.st - which is only available for R >= 3.1.0.

My question is - how do I work with HuGene 2.0 ST arrays in Bioconductor?  I just want to extract RMA values for input into limma.

Many thanks

Mick

affy oligo • 2.3k views
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@benilton-carvalho-1375
Last seen 4.7 years ago
Brazil/Campinas/UNICAMP
Can you please describe what error you get when installing oligo on R-3.1.1? I have it running just fine on R 3.1.1 (on Mac OS X) and on R 3.1.2 (on Ubuntu). On Mon Jan 19 2015 at 3:15:43 PM WATSON Mick [bioc] < noreply@bioconductor.org> wrote: > Activity on a post you are following on support.bioconductor.org > > User WATSON Mick <https: support.bioconductor.org="" u="" 5575=""/> wrote Question: > Reading Affymetrix data from pd.hugene.2.0.st using oligo/affy > <https: support.bioconductor.org="" p="" 64214=""/>: > > Hi > > Please accept my apologies if this is obvious, it's been some time since I > have worked with microarray data. > > I have some Affymetrix CEL files that use the HuGene 2.0 ST design ( > http://www.bioconductor.org/packages/release/data/annotation/html/pd.hugene.2.0.st.html) > . > > Running R 3.1.1, I tried reading these using the Affy package, but Affy > refused and recommended I use the oligo or xps package. But neither oligo > nor xps install in R 3.1.1 so I downgraded to R 2.15.2. > > Now I can install oligo, but it asks for the annotation package - > pd.hugene.2.0.st - which is only available for R >= 3.1.0. > > My question is - how do I work with HuGene 2.0 ST arrays in Bioconductor? > I just want to extract RMA values for input into limma. > > Many thanks > > Mick > ------------------------------ > > You may reply via email or visit Reading Affymetrix data from pd.hugene.2.0.st using oligo/affy >
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WATSON Mick ▴ 50
@watson-mick-5575
Last seen 9.9 years ago
United Kingdom
Hi Thanks for the reply. Using biocLite.R, the function said that oligo was not available for R 3.1.1. Do I need to grab the tar.gz instead? Cheers Mick
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It's hard to say without further details. If you send the (full) results of the following commands, I'd be able to help you out. Start a brand-new R session and type: sessionInfo() source('http://www.bioconductor.org/biocLite.R') biocLite('oligo') Thank you On Mon Jan 19 2015 at 6:40:26 PM WATSON Mick [bioc] < noreply@bioconductor.org> wrote: > Activity on a post you are following on support.bioconductor.org > > User WATSON Mick <https: support.bioconductor.org="" u="" 5575=""/> wrote Answer: > Reading Affymetrix data from pd.hugene.2.0.st using oligo/affy > <https: support.bioconductor.org="" p="" 64214="" #64218="">: > > Hi Thanks for the reply. Using biocLite.R, the function said that oligo > was not available for R 3.1.1. Do I need to grab the tar.gz instead? Cheers > Mick > ------------------------------ > > You may reply via email or visit > A: Reading Affymetrix data from pd.hugene.2.0.st using oligo/affy >
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Biologist ▴ 120
@biologist-9801
Last seen 4.7 years ago

Hi,

I have some Affymetrix CEL files that use the HuGene 2.0 ST design.

Can I use oligo package for RNA degradation plot?

Thanks

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