beadarray - QC file loads but doesn't appear in ExpressionSetIllumina object
0
0
Entering edit mode
meeta.mistry ▴ 30
@meetamistry-7355
Last seen 2.4 years ago
United States

Hello;

I am using the readBeadSummary function to load in my data using two files: data and control probe files (as shown in the vignette example). While I can run it with no errors when I view the ExpressionSet object there appears to be no QC data loaded under 'QC Information'  (see below).  Where is the data stored?

Thanks, Meeta

datafile <- file.path(metaDir, "sample_probe_profile.csv")
qcfile <- file.path(metaDir, "control_probes.csv")

beadData <- readBeadSummaryData(dataFile = datafile, qcFile = qcfile, sep=",", 
                   ProbeID = "PROBE_ID", skip = 0, 
                   columns = list(exprs ="AVG_Signal", 
                   Detection = "Detection_Pval", se.exprs = "BEAD_STDERR",
                   nObservations = "Avg_NBEADS"),
                   qc.skip = 0, qc.sep =",", controlID = "ProbeID", 
                   qc.columns = list(exprs = "AVG_Signal", Detection ="Detection_Pval"),
                   annoCols=c("PROBE_ID", "SYMBOL","ENTREZ_GENE_ID","CHROMOSOME"))

 

> beadData
ExpressionSetIllumina (storageMode: list)
assayData: 26526 features, 24 samples 
  element names: exprs, se.exprs, nObservations, Detection 
protocolData: none
phenoData
  rowNames: 9974433081_A 9974433081_B ... 9982502025_H (24 total)
  varLabels: sampleID
  varMetadata: labelDescription
featureData
  featureNames: ILMN_2896528 ILMN_2721178 ... 7650601 (26526 total)
  fvarLabels: ProbeID PROBE_ID ... Status (6 total)
  fvarMetadata: labelDescription
experimentData: use 'experimentData(object)'
Annotation:  
QC Information
 Available Slots:  
  QC Items: 
  sampleNames: 
beadarray qc • 1.4k views
ADD COMMENT

Login before adding your answer.

Traffic: 792 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6