Question

Deseq2 and gene clustering

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aristotele_m ▴ 40

@aristotele_m-6821

Last seen 7.3 years ago

Italy

Hi!

I use this tutorial on this site http://www.bioconductor.org/help/workflows/rnaseqGene/:

library("genefilter")
topVarGenes <- head(order(-rowVars(assay(rld))),35)

Why -rowVars?

deseq2 clustering • 1.4k views

ADD COMMENT • link updated 9.5 years ago by Michael Love 42k • written 9.5 years ago by aristotele_m ▴ 40

score 0 · Answer 1 · 2015-03-15

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Michael Love 42k

@mikelove

Last seen 1 day ago

United States

See the man page for the function if you have questions about its behavior: ?order. The default for order() is small to large so adding a minus sign sorts large to small.

ADD COMMENT • link 9.5 years ago Michael Love 42k