hiii 

 try to get secondary prediction by R language and i run the code 

 predictPROTEUS from BioSeqClass in  R language

 PROTEUS = predictPROTEUS(proteinSeq[1:2],proteus2.organism="euk")
Error in file(file, "rt") : cannot open the connection
In addition: Warning messages:
1: running command 'perl C:\Users\D58B~1\AppData\Local\Temp\Rtmpu8B2vI\file1728128132c4.pl' had status 127 
2: In file(file, "rt") :
  cannot open file 'C:\Users\D58B~1\AppData\Local\Temp\Rtmpu8B2vI\file1728128132c4.proteus2': No such file or directory

any suggestion