Hi, I have a list of genes from affymetrix and the ID's come out after normalizing the CEL files , how can i define their pathways from KEGG, can we do that using KEGGREST.

Dear navieng2uk,

could you provide some more information regarding the specific Affymetrix platform/annotation database you use for your analysis and what is your purpose ? I mean you want to perform some king of gene-set(pathway) analysis with some list of DEG genes?

I havent used KEGGREST, but the below vignette may be useful

http://www.bioconductor.org/packages/release/bioc/vignettes/KEGGREST/inst/doc/KEGGREST-vignette.html

Dear navieng2uk,

you should also try this platform:

http://david.abcc.ncifcrf.gov/

Go to "Start analysis" bookmark, upload your list and select KEGG under "function annotation clustering".

Best regards,
Dimitri