Hi,

I try to DESeq find the differentially expressed genes in two samples.

The result is all the pvalue>0.05, and qvalue=1.

Any suggestion or advice?

Thanks for great help!

Yue

Try DESeq2. This is the currently supported version of the DESeq method. Still you might have no significant genes because you have no replicates and hence very little power to detect differences. Suggestion: sequence one more sample for each condition.

http://bioconductor.org/packages/DESeq2

Thanks, I will try DESeq2.

Thanj you for your help!