Question: Undefined columns selected in codelink R package.
0
gravatar for Agaz Hussain Wani
4.4 years ago by
India
Agaz Hussain Wani260 wrote:

I am trying to use raw data sample to generate expression values and SNR. I used GSM240525 raw file, which goes very smooth. I tried to use other sample GSM718640 to get expression and SNR.

Error in `[.data.frame`(data, , "Spot_mean") : undefined columns selected

 

I am not sure what is going wrong .I guess that some information is missing. If Its not a valid codelink file, why not an understandable error message??.

 

 

codelink R txt • 979 views
ADD COMMENTlink modified 4.4 years ago • written 4.4 years ago by Agaz Hussain Wani260

The error is a standard R error message and is self-explanatory: you have tried to access a column called "Spot_mean" in a data.frame and the column does not exists. Now, why did you get that error I cannot tell, since you did not provide the code and conditions necessary to reproduce your error. Basically, I have no idea what you did so can't help you. So, please, provide, as usual, a reproducible example with the code that triggers this error and I might be able to figure out what went wrong.

ADD REPLYlink written 4.4 years ago by Diego Diez750

You are right about the standard R error message but here i am using codelink package to process the raw data . Out of the two sample files pasted above, only one is valid codelink output file while the other is missing some information. It should display a message that file is not a valid codelink file other than throwing a general error message which is some times frustrating.

I am not sure which information is needed to process the raw codelink files but as per the error message Spot_mean is a must. Your can compare the two sample files and check out.

The code

codset = readCodelinkSet(filename)

throws an error with the second sample.
ADD REPLYlink written 4.4 years ago by Agaz Hussain Wani260

 

I may consider improving the error message in such situations, but please, alway include the relevant information when posting a problem. Otherwise it is hard to understand what the problem is. Since you have been using codelink and GEOquery in some other posts there is not explicit reason why I should "know" what you did.

Regarding your particular problem, some Codelink files do not contain all the information, as we have discussed before. In this case it is missing Spot_mean. You can use instead Raw_intensity (pass type =  "Raw") or Normalized_intensity (pass type = "Norm") to load the data (this is briefly mentioned in the vignette). But right now this fails due to the weight function that I added last year.

The problem, as usual, is the inconsistency in the Codelink file format. So far I have not had any reports of this because no one had used files for different studies before. So you get to be my testing subject. Thank you for the report- I will add this to the list of fixes and will let you know when it is solved.

ADD REPLYlink modified 4.4 years ago • written 4.4 years ago by Diego Diez750
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