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Hello,
I'm following the RNA-seq workflow : gene-level exploratory analysis and differential expression.
in this step, the error appear,
> library("GenomicFeatures") > gtffile <- file.path(dir,"Homo_sapiens.GRCh37.75_subset.gtf") > txdb <- makeTranscriptDbFromGFF(gtffile, format="gtf") Error in nchar(attrs) : could not find symbol "keepNA" in environment of the generic function In addition: Warning message: 'makeTranscriptDbFromGFF' is deprecated. Use 'makeTxDbFromGFF' instead. See help("Deprecated") > txdb <- makeTxDbFromGFF(gtffile, format="gtf") Error in nchar(attrs) : could not find symbol "keepNA" in environment of the generic function.
I am a newcomer trying to analysis RNASeq data. Can someone help please!
Thanks for your help. Updating to R-3.2.1 solves the problem.