Champ.lasso error @ dmr.beta.means[,22:24]
2
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Entering edit mode
zhourudy2 ▴ 10
@zhourudy2-8428
Last seen 9.4 years ago
Canada

Package source v1.6.0

      champ.lasso(fromFile=T,limma=annottated,beta.norm=batchNorm$beta,batchDone=TRUE,pd=myLoad$pd,image=F,bedFile=FALSE,resultsDir=getwd())
trace: champ.lasso(fromFile = T, limma = annottated, beta.norm = batchNorm$beta, 
    batchDone = TRUE, pd = myLoad$pd, image = F, bedFile = FALSE, 
    resultsDir = getwd())
Run Probe Lasso DMR Hunter
Error in `[.data.frame`(dmr.beta.means, , 22:24, ) : 
  undefined columns selected

 

Using default champ.lasso and limma returns this error, was able to remedy by changing column reference to [,17:19]. Can only guess it is referring to the wrong columns in the limma results obtained from champ.MVP

 

Best,

Rudy

> sessionInfo()
R version 3.2.1 (2015-06-18)
Platform: x86_64-apple-darwin13.4.0 (64-bit)
Running under: OS X 10.10.4 (Yosemite)

locale:
[1] en_CA.UTF-8/en_CA.UTF-8/en_CA.UTF-8/C/en_CA.UTF-8/en_CA.UTF-8

attached base packages:
[1] stats4    parallel  stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] IlluminaHumanMethylation450kmanifest_0.4.0         ChAMP_1.6.0                                        Illumina450ProbeVariants.db_1.4.0                 
 [4] ChAMPdata_1.6.0                                    wateRmelon_1.8.0                                   IlluminaHumanMethylation450kanno.ilmn12.hg19_0.2.1
 [7] ROC_1.44.0                                         lumi_2.20.2                                        methylumi_2.14.0                                  
[10] minfi_1.14.0                                       bumphunter_1.8.0                                   locfit_1.5-9.1                                    
[13] iterators_1.0.7                                    foreach_1.4.2                                      Biostrings_2.36.1                                 
[16] XVector_0.8.0                                      lattice_0.20-31                                    FDb.InfiniumMethylation.hg19_2.2.0                
[19] org.Hs.eg.db_3.1.2                                 RSQLite_1.0.0                                      DBI_0.3.1                                         
[22] TxDb.Hsapiens.UCSC.hg19.knownGene_3.1.2            GenomicFeatures_1.20.1                             AnnotationDbi_1.30.1                              
[25] GenomicRanges_1.20.5                               GenomeInfoDb_1.4.1                                 IRanges_2.2.5                                     
[28] S4Vectors_0.6.1                                    ggplot2_1.0.1                                      reshape2_1.4.1                                    
[31] scales_0.2.5                                       Biobase_2.28.0                                     BiocGenerics_0.14.0                               
[34] matrixStats_0.14.2                                 limma_3.24.13                                      plyr_1.8.3                                        

loaded via a namespace (and not attached):
 [1] nlme_3.1-121            bitops_1.0-6            RColorBrewer_1.1-2      tools_3.2.1             doRNG_1.6               nor1mix_1.2-0          
 [7] affyio_1.36.0           KernSmooth_2.23-15      mgcv_1.8-6              colorspace_1.2-6        DNAcopy_1.42.0          base64_1.1             
[13] preprocessCore_1.30.0   RPMM_1.20               pkgmaker_0.22           rtracklayer_1.28.6      genefilter_1.50.0       quadprog_1.5-5         
[19] affy_1.46.1             stringr_1.0.0           digest_0.6.8            Rsamtools_1.20.4        illuminaio_0.10.0       siggenes_1.42.0        
[25] GEOquery_2.34.0         impute_1.42.0           BiocInstaller_1.18.3    mclust_5.0.2            BiocParallel_1.2.8      RCurl_1.95-4.7         
[31] magrittr_1.5            futile.logger_1.4.1     Matrix_1.2-2            Rcpp_0.11.6             munsell_0.4.2           proto_0.3-10           
[37] stringi_0.5-5           nleqslv_2.8             MASS_7.3-42             zlibbioc_1.14.0         grid_3.2.1              splines_3.2.1          
[43] multtest_2.24.0         annotate_1.46.0         beanplot_1.2            rngtools_1.2.4          marray_1.46.0           codetools_0.2-11       
[49] biomaRt_2.24.0          futile.options_1.0.0    XML_3.98-1.3            lambda.r_1.1.7          gtable_0.1.2            reshape_0.8.5          
[55] xtable_1.7-4            survival_2.38-3         GenomicAlignments_1.4.1 registry_0.3            cluster_2.0.2           sva_3.14.0  

ChAMP • 1.4k views
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1
Entering edit mode
yourell.alan ▴ 10
@yourellalan-8298
Last seen 9.3 years ago
United Kingdom

Hi Rudy,

I have got the same error except I am not using the output from champ.MVP. Instead I am using the output from Limma topTable. Any ideas on what might be happening? I assume it is searching for a column that doesn't exist in data. Am I even using the right data?

 

Any help much appreciated,

Alan

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0
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david.watson ▴ 30
@davidwatson-9545
Last seen 5.9 years ago
Queen Mary University of London

There's an extra comma in the source code for champ.lasso at line 211 defining dmr.beta.means. You can tell from the error message, where there are commas before and after the column specification. Try deleting the second comma and rerunning. 

 

Cheers,

David

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