Off topic:Pairwise comparasion for multiple conditions using DEXSeq
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@chentong_biology-9142
Last seen 7.1 years ago
China

Hi, I have a question about DEXSeq package for analyzing multiple samples. Like the sample information listed below. I have six samples loaded as DEXSeqDataSets. Default, every sample will be compared with the first sample known as Col0 here. 

I wonder if there is any function to extract pairwise comparasion results as res <- results(dds, contrast=c("conditions", "Col3", "Col24")) in DESeq2

Thanks!

Tong Chen

 

> sample
        conditions cell time
Col0_1        Col0  Col    0
Col0_2        Col0  Col    0
Col3_1        Col3  Col    3
Col3_2        Col3  Col    3
Col24_1      Col24  Col   24
Col24_2      Col24  Col   24
cbf0_1        cbf0  cbf    0
cbf0_2        cbf0  cbf    0
cbf3_1        cbf3  cbf    3
cbf3_2        cbf3  cbf    3
cbf24_1      cbf24  cbf   24
cbf24_2      cbf24  cbf   24

> colData(dxd)
DataFrame with 24 rows and 6 columns
      sample conditions     cell     time     exon sizeFactor
    <factor>   <factor> <factor> <factor> <factor>  <numeric>
1     Col0_1       Col0      Col        0     this  0.9126171
2     Col0_2       Col0      Col        0     this  0.9458306
3     Col3_1       Col3      Col        3     this  0.8914356
4     Col3_2       Col3      Col        3     this  1.2490106
5    Col24_1      Col24      Col       24     this  0.9687948
...      ...        ...      ...      ...      ...        ...
20    cbf0_2       cbf0      cbf        0   others  1.0687401
21    cbf3_1       cbf3      cbf        3   others  0.9824029
22    cbf3_2       cbf3      cbf        3   others  1.0701895
23   cbf24_1      cbf24      cbf       24   others  0.9257264
24   cbf24_2      cbf24      cbf       24   others  1.1723387

 

 

> dds <- DEXSeqResults(dxd)
> dds
                                         <numeric> <numeric> <numeric>
XLOC_000002+XLOC_004161:E001             10.882663  10.44266 10.388549
XLOC_000002+XLOC_004161:E002             20.561282  20.03267 22.448874
XLOC_000002+XLOC_004161:E003              9.510509  10.14679  9.860588
XLOC_000002+XLOC_004161:E004             10.871525  11.25257 10.929886
XLOC_000002+XLOC_004161:E005             14.204665  13.51434 13.703109
...                                            ...       ...       ...
XLOC_032817+XLOC_032818+XLOC_032742:E008  4.024608  4.100783  2.736283
XLOC_032817+XLOC_032818+XLOC_032742:E009  2.848699  2.606964  1.367766
XLOC_032820:E001                                NA        NA        NA
XLOC_032821:E001                                NA        NA        NA
XLOC_032823:E001                                NA        NA        NA
                                              cbf0      cbf3      cbf24
                                         <numeric> <numeric>  <numeric>
XLOC_000002+XLOC_004161:E001              8.553736  9.334228   9.814653
XLOC_000002+XLOC_004161:E002             18.986580 19.484306  23.109959
XLOC_000002+XLOC_004161:E003              9.409119  9.513795  11.259645
XLOC_000002+XLOC_004161:E004             10.103124 11.030351  12.261620
XLOC_000002+XLOC_004161:E005             12.996807 13.718990  15.307747
...                                            ...       ...        ...
XLOC_032817+XLOC_032818+XLOC_032742:E008  3.850081  3.421292 4.39521544
XLOC_032817+XLOC_032818+XLOC_032742:E009  2.061519  1.715996 0.05112219
XLOC_032820:E001                                NA        NA         NA
XLOC_032821:E001                                NA        NA         NA
XLOC_032823:E001                                NA        NA         NA
                                         log2fold_Col3_Col0 log2fold_Col24_Col0
                                                  <numeric>           <numeric>
XLOC_000002+XLOC_004161:E001                    -0.05954207        -0.067037538
XLOC_000002+XLOC_004161:E002                    -0.03757565         0.126712863
XLOC_000002+XLOC_004161:E003                     0.09342863         0.052151148
XLOC_000002+XLOC_004161:E004                     0.04970028         0.007724016
XLOC_000002+XLOC_004161:E005                    -0.07187362        -0.051861600
...                                                     ...                 ...
XLOC_032817+XLOC_032818+XLOC_032742:E008         0.02705116          -0.5566307
XLOC_032817+XLOC_032818+XLOC_032742:E009        -0.12793236          -1.0584817
XLOC_032820:E001                                         NA                  NA
XLOC_032821:E001                                         NA                  NA
XLOC_032823:E001                                         NA                  NA
                                         log2fold_cbf0_Col0 log2fold_cbf3_Col0
                                                  <numeric>          <numeric>
XLOC_000002+XLOC_004161:E001                    -0.34740516      -0.2214290920
XLOC_000002+XLOC_004161:E002                    -0.11495013      -0.0776176400
XLOC_000002+XLOC_004161:E003                    -0.01546295       0.0004983823
XLOC_000002+XLOC_004161:E004                    -0.10575280       0.0209244683
XLOC_000002+XLOC_004161:E005                    -0.12820759      -0.0501904871
...                                                     ...                ...
XLOC_032817+XLOC_032818+XLOC_032742:E008        -0.06395915         -0.2343070
XLOC_032817+XLOC_032818+XLOC_032742:E009        -0.46659536         -0.7312565
XLOC_032820:E001                                         NA                 NA
XLOC_032821:E001                                         NA                 NA
XLOC_032823:E001                                         NA                 NA
                                         log2fold_cbf24_Col0
                                                   <numeric>
XLOC_000002+XLOC_004161:E001                      -0.1490225
XLOC_000002+XLOC_004161:E002                       0.1685845
XLOC_000002+XLOC_004161:E003                       0.2435668
XLOC_000002+XLOC_004161:E004                       0.1735953
XLOC_000002+XLOC_004161:E005                       0.1078971
...                                                      ...
XLOC_032817+XLOC_032818+XLOC_032742:E008           0.1270858
XLOC_032817+XLOC_032818+XLOC_032742:E009          -5.8002097
XLOC_032820:E001                                          NA
XLOC_032821:E001                                          NA
XLOC_032823:E001                                          NA

 

 

DEXSeq contrast • 643 views
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