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@tarekmohamed-9489
Last seen 2.1 years ago

ear Michael,

 

My name is Tarek , I am using QuasR package for RNAseq analysis ( I have 12 samples of paired end reads). I

did alignment using

sampleFile <- "2_DOX_RNAseq.txt"
genomeFile<-"BSgenome.Hsapiens.NCBI.GRCh38"
proj <- qAlign(sampleFile, genomeFile)

It was successful for 8 samples , then I got an error message 

" one node produced an error: Error on FSMMJ02UCX1 processing sample C:\Users\tmm447\AppData\Local\Temp\RtmpkLS7LW\CON3_0uM_1.fastq.gz1ae8210858ab.fastq : bowtie failed to perform the alignments"  

 

I re-did the analysis and I got the same errorr again. 

How can I solve this issue and why it worked successfully for 8 samples then it was stuck.

 

Thanks

Tarek

QuasR • 710 views
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@hotz-hans-rudolf-3951
Last seen 4 months ago
Switzerland

see my answer for your other post :  (QuasR Error) Error in checkForRemoteErrors(val) : one node produced an error: bowtie failed to perform the alignments

 

Hans-Rudolf

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