My data is setup as in the MetaDE vignette:
-
gene_tstats
&gene_pvals
are matrices with rownames corresponding to gene names and moderated t-statistic / p-values for each study. -
gene_tstats
andgene_pvals
have the same column names and dimensions (20917 * 3) and no NA values (although this shouldn't matter?)
data <- list(stat=gene_tstats, p=gene_pvals) metade <- MetaDE.pvalue(data, meta.method='AW.OC')
I get this error:
Warning message: In get.AW.OC(x$p, x$bp): there're no parametric results for Pearson's method,we will use simulation to estimate the p valuesWarning message: In matrix(Vb[, i], nr, np/nr): data length exceeds size of matrixWarning message: In matrix(Vb[, i], nr, np/nr): data length exceeds size of matrixWarning message: In matrix(Vb[, i], nr, np/nr): data length exceeds size of matrixWarning message: In matrix(Vb[, i], nr, np/nr): data length exceeds size of matrixWarning message: In matrix(Vb[, i], nr, np/nr): data length exceeds size of matrixWarning message: In matrix(Vb[, i], nr, np/nr): data length exceeds size of matrixWarning message: In matrix(Vb[, i], nr, np/nr): data length exceeds size of matrix Error in xj[i]: invalid subscript type 'list'
If I use a subset of my data, there are no error messages:
data100 <- list(stat=gene_tstats[1:100,], p=gene_pvals[1:100,]) metade <- MetaDE.pvalue(data100, meta.method='AW.OC') Warning message: In get.AW.OC(x$p, x$bp): there're no parametric results for Pearson's method,we will use simulation to estimate the p values
Larger subsets produce different warning messages but do return a result successfully:
data2000 <- list(stat=gene_tstats[1:2000,], p=gene_pvals[1:2000,]) metade <- MetaDE.pvalue(data2000, meta.method='AW.OC') Warning message: In get.AW.OC(x$p, x$bp): there're no parametric results for Pearson's method,we will use simulation to estimate the p valuesWarning message: In matrix(Vb[, i], nr, np/nr): data length [5000] is not a sub-multiple or multiple of the number of rows [2000]Warning message: In matrix(Vb[, i], nr, np/nr): data length [5000] is not a sub-multiple or multiple of the number of rows [2000]Warning message: In matrix(Vb[, i], nr, np/nr): data length [5000] is not a sub-multiple or multiple of the number of rows [2000]Warning message: In matrix(Vb[, i], nr, np/nr): data length [5000] is not a sub-multiple or multiple of the number of rows [2000]Warning message: In matrix(Vb[, i], nr, np/nr): data length [5000] is not a sub-multiple or multiple of the number of rows [2000]Warning message: In matrix(Vb[, i], nr, np/nr): data length [5000] is not a sub-multiple or multiple of the number of rows [2000]Warning message: In matrix(Vb[, i], nr, np/nr): data length [5000] is not a sub-multiple or multiple of the number of rows [2000]
This only works up to a point:
data10000 <- list(stat=gene_tstats[1:10000,], p=gene_pvals[1:10000,]) metade <- MetaDE.pvalue(data10000, meta.method='AW.OC') Warning message: In get.AW.OC(x$p, x$bp): there're no parametric results for Pearson's method,we will use simulation to estimate the p valuesWarning message: In matrix(Vb[, i], nr, np/nr): data length exceeds size of matrixWarning message: In matrix(Vb[, i], nr, np/nr): data length exceeds size of matrixWarning message: In matrix(Vb[, i], nr, np/nr): data length exceeds size of matrixWarning message: In matrix(Vb[, i], nr, np/nr): data length exceeds size of matrixWarning message: In matrix(Vb[, i], nr, np/nr): data length exceeds size of matrixWarning message: In matrix(Vb[, i], nr, np/nr): data length exceeds size of matrixWarning message: In matrix(Vb[, i], nr, np/nr): data length exceeds size of matrix Error in xj[i]: invalid subscript type 'list'
I't seems to be something related to the number of genes I want to do a meta analysis for. Suggestions on how to resolve (other than to subset my data)?