Processing iScan raw data without perBeadFile.txt
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Entering edit mode
aizhang • 0
@aizhang-10020
Last seen 8.7 years ago

I'm trying to process iScan raw data with beadarray, but it seems that I'm missing perBeadFile.txt in order to use the package.

The list of files I got from the scanner including . I've seen this problem posted before, but couldn't find a solution. Can anyone point me to a right direction or another package that can process the raw data with the file types that I have? Many thanks!

[1] "200143170019.sdf"                                      "200143170019_A-Swath1_Grn.jpg"                        
  [3] "200143170019_A-Swath1_Grn.locs"                        "200143170019_A-Swath2_Grn.jpg"                        
  [5] "200143170019_A-Swath2_Grn.locs"                        "200143170019_A-Swath3_Grn.jpg"                        
  [7] "200143170019_A-Swath3_Grn.locs"                        "200143170019_A.dmap.gz"                               
  [9] "200143170019_A_Focus_scan#1_swath#1_point#1_try#1.jpg" "200143170019_A_Green.xml"                             
 [11] "200143170019_A_Grn.idat"                               "200143170019_A_Red.xml"                               
 [13] "200143170019_B-Swath1_Grn.jpg"                         "200143170019_B-Swath1_Grn.locs"                       
 [15] "200143170019_B-Swath2_Grn.jpg"                         "200143170019_B-Swath2_Grn.locs"                       
 [17] "200143170019_B-Swath3_Grn.jpg"                         "200143170019_B-Swath3_Grn.locs"                       
 [19] "200143170019_B.dmap.gz"                                "200143170019_B_Focus_scan#1_swath#1_point#1_try#1.jpg"
 [21] "200143170019_B_Green.xml"                              "200143170019_B_Grn.idat"                              
 [23] "200143170019_B_Red.xml"                                "200143170019_C-Swath1_Grn.jpg"                        
 [25] "200143170019_C-Swath1_Grn.locs"                        "200143170019_C-Swath2_Grn.jpg"                        
 [27] "200143170019_C-Swath2_Grn.locs"                        "200143170019_C-Swath3_Grn.jpg"                        
 [29] "200143170019_C-Swath3_Grn.locs"                        "200143170019_C.dmap.gz"                               
 [31] "200143170019_C_Focus_scan#1_swath#1_point#1_try#1.jpg" "200143170019_C_Green.xml"                             
 [33] "200143170019_C_Grn.idat"                               "200143170019_C_Red.xml"                               
 [35] "200143170019_CheckSum.txt"                             "200143170019_D-Swath1_Grn.jpg"                        
 [37] "200143170019_D-Swath1_Grn.locs"                        "200143170019_D-Swath2_Grn.jpg"                        
 [39] "200143170019_D-Swath2_Grn.locs"                        "200143170019_D-Swath3_Grn.jpg"                        
 [41] "200143170019_D-Swath3_Grn.locs"                        "200143170019_D.dmap.gz"                               
 [43] "200143170019_D_Focus_scan#1_swath#1_point#1_try#1.jpg" "200143170019_D_Green.xml"                             
 [45] "200143170019_D_Grn.idat"                               "200143170019_D_Red.xml"                               
 [47] "200143170019_E-Swath1_Grn.jpg"                         "200143170019_E-Swath1_Grn.locs"                       
 [49] "200143170019_E-Swath2_Grn.jpg"                         "200143170019_E-Swath2_Grn.locs"                       
 [51] "200143170019_E-Swath3_Grn.jpg"                         "200143170019_E-Swath3_Grn.locs"                       
 [53] "200143170019_E.dmap.gz"                                "200143170019_E_Focus_scan#1_swath#1_point#1_try#1.jpg"
 [55] "200143170019_E_Green.xml"                              "200143170019_E_Grn.idat"                              
 [57] "200143170019_E_Red.xml"                                "200143170019_F-Swath1_Grn.jpg"                        
 [59] "200143170019_F-Swath1_Grn.locs"                        "200143170019_F-Swath2_Grn.jpg"                        
 [61] "200143170019_F-Swath2_Grn.locs"                        "200143170019_F-Swath3_Grn.jpg"                        
 [63] "200143170019_F-Swath3_Grn.locs"                        "200143170019_F.dmap.gz"                               
 [65] "200143170019_F_Focus_scan#1_swath#1_point#1_try#1.jpg" "200143170019_F_Green.xml"                             
 [67] "200143170019_F_Grn.idat"                               "200143170019_F_Red.xml"                               
 [69] "200143170019_G-Swath1_Grn.jpg"                         "200143170019_G-Swath1_Grn.locs"                       
 [71] "200143170019_G-Swath2_Grn.jpg"                         "200143170019_G-Swath2_Grn.locs"                       
 [73] "200143170019_G-Swath3_Grn.jpg"                         "200143170019_G-Swath3_Grn.locs"                       
 [75] "200143170019_G.dmap.gz"                                "200143170019_G_Focus_scan#1_swath#1_point#1_try#1.jpg"
 [77] "200143170019_G_Green.xml"                              "200143170019_G_Grn.idat"                              
 [79] "200143170019_G_Red.xml"                                "200143170019_H-Swath1_Grn.jpg"                        
 [81] "200143170019_H-Swath1_Grn.locs"                        "200143170019_H-Swath2_Grn.jpg"                        
 [83] "200143170019_H-Swath2_Grn.locs"                        "200143170019_H-Swath3_Grn.jpg"                        
 [85] "200143170019_H-Swath3_Grn.locs"                        "200143170019_H.dmap.gz"                               
 [87] "200143170019_H_Focus_scan#1_swath#1_point#1_try#1.jpg" "200143170019_H_Green.xml"                             
 [89] "200143170019_H_Grn.idat"                               "200143170019_H_Red.xml"                               
 [91] "200143170019_I-Swath1_Grn.jpg"                         "200143170019_I-Swath1_Grn.locs"                       
 [93] "200143170019_I-Swath2_Grn.jpg"                         "200143170019_I-Swath2_Grn.locs"                       
 [95] "200143170019_I-Swath3_Grn.jpg"                         "200143170019_I-Swath3_Grn.locs"                       
 [97] "200143170019_I.dmap.gz"                                "200143170019_I_Focus_scan#1_swath#1_point#1_try#1.jpg"
 [99] "200143170019_I_Green.xml"                              "200143170019_I_Grn.idat"                              
[101] "200143170019_I_Red.xml"                                "200143170019_J-Swath1_Grn.jpg"                        
[103] "200143170019_J-Swath1_Grn.locs"                        "200143170019_J-Swath2_Grn.jpg"                        
[105] "200143170019_J-Swath2_Grn.locs"                        "200143170019_J-Swath3_Grn.jpg"                        
[107] "200143170019_J-Swath3_Grn.locs"                        "200143170019_J.dmap.gz"                               
[109] "200143170019_J_Focus_scan#1_swath#1_point#1_try#1.jpg" "200143170019_J_Green.xml"                             
[111] "200143170019_J_Grn.idat"                               "200143170019_J_Red.xml"                               
[113] "200143170019_K-Swath1_Grn.jpg"                         "200143170019_K-Swath1_Grn.locs"                       
[115] "200143170019_K-Swath2_Grn.jpg"                         "200143170019_K-Swath2_Grn.locs"                       
[117] "200143170019_K-Swath3_Grn.jpg"                         "200143170019_K-Swath3_Grn.locs"                       
[119] "200143170019_K.dmap.gz"                                "200143170019_K_Focus_scan#1_swath#1_point#1_try#1.jpg"
[121] "200143170019_K_Green.xml"                              "200143170019_K_Grn.idat"                              
[123] "200143170019_K_Red.xml"                                "200143170019_L-Swath1_Grn.jpg"                        
[125] "200143170019_L-Swath1_Grn.locs"                        "200143170019_L-Swath2_Grn.jpg"                        
[127] "200143170019_L-Swath2_Grn.locs"                        "200143170019_L-Swath3_Grn.jpg"                        
[129] "200143170019_L-Swath3_Grn.locs"                        "200143170019_L.dmap.gz"                               
[131] "200143170019_L_Focus_scan#1_swath#1_point#1_try#1.jpg" "200143170019_L_Green.xml"                             
[133] "200143170019_L_Grn.idat"                               "200143170019_L_Red.xml"                               
[135] "200143170019_qc.txt"                                   "AutoCenter_focus_fiducial#1_alt#1.jpg"                
[137] "AutoCenter_focus_fiducial#2_alt#1.jpg"                 "AutoCenter_focus_fiducial#3_alt#1.jpg"                
[139] "AutoTilt_focus_fiducial#1_alt#1_focus#1.jpg"           "AutoTilt_focus_fiducial#1_alt#1_focus#1_old0.jpg"     
[141] "AutoTilt_focus_fiducial#2_alt#1_focus#1.jpg"           "AutoTilt_focus_fiducial#2_alt#1_focus#1_old0.jpg"     
[143] "AutoTilt_focus_fiducial#2_alt#1_focus#2.jpg"           "AutoTilt_focus_fiducial#3_alt#1_focus#1.jpg"          
[145] "AutoTilt_focus_fiducial#3_alt#1_focus#1_old0.jpg"      "Effective.cfg"                                        
[147] "Metrics.txt"         
microarray beadarray • 1.1k views
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Entering edit mode
Mike Smith ★ 6.6k
@mike-smith
Last seen 46 minutes ago
EMBL Heidelberg

Unfortunately, I don't think you are able to perform a bead-level analysis with the file you have.  The locs files will give you you the locations of beads in the scanned images, but they have no indication of the bead's identity, while the idat files contain only summarised bead-type data.

The perBeadFile.txt files are really the only way to access useful data at the bead-level, and I have never seen any way to obtain them if the option wasn't selected when the arrays were scanned.

In your situation, I would advise you work with lumi or limma and work with the summarised information in the idat files.

 

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