I run DEXSeq on my data. I have 16 individuals in each conditions (2 conditions). After computing the dispersions with estimateDispersions( dxd ), I plotted the graph plotDispEsts( dxd ) and I obtain this :
I tried the others ways to fit (local and mean) but I obtained the same kind of shrinkage.
Can you please tell me if my graph seems to be ok ? And if it's not, what it could be ?
Moreover, is there any way to recover the normalized data ?
Thanks for you help,