methyAnalysis error with example data
0
0
Entering edit mode
Tom ▴ 10
@tom-10763
Last seen 5.3 years ago

Hello,

 

I'm working on illumina methylation array data. Focusing on the methylation pattern at 11p15.5, a imprinted region, I plan to use "methyAnalysis" to draw a heatmap by chromosome location.

Before actually analysing my data, I first tried the example codes and got the following error:

> library(methyAnalysis)
Loading required package: grid
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:parallel':

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ, clusterExport,
    clusterMap, parApply, parCapply, parLapply, parLapplyLB, parRapply,
    parSapply, parSapplyLB

The following objects are masked from 'package:stats':

    IQR, mad, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, append, as.data.frame,
    cbind, colnames, do.call, duplicated, eval, evalq, get, grep, grepl,
    intersect, is.unsorted, lapply, lengths, mapply, match, mget, order, paste,
    pmax, pmax.int, pmin, pmin.int, rank, rbind, rownames, sapply, setdiff, sort,
    table, tapply, union, unique, unsplit

Loading required package: IRanges
Loading required package: S4Vectors
Loading required package: stats4

Attaching package: 'S4Vectors'

The following objects are masked from 'package:base':

    colMeans, colSums, expand.grid, rowMeans, rowSums

Loading required package: GenomeInfoDb
Loading required package: GenomicRanges
Loading required package: Biobase
Welcome to Bioconductor

    Vignettes contain introductory material; view with 'browseVignettes()'. To
    cite Bioconductor, see 'citation("Biobase")', and for packages
    'citation("pkgname")'.

Loading required package: org.Hs.eg.db
Loading required package: AnnotationDbi

Setting options('download.file.method.GEOquery'='auto')
Setting options('GEOquery.inmemory.gpl'=FALSE)
No methods found in "GenomicRanges" for requests: overlapsAny
No methods found in "genoset" for requests: rowRanges, rowRanges<-, toGenomeOrder
Warning messages:
1: multiple methods tables found for 'colMeans' 
2: multiple methods tables found for 'colSums' 
3: multiple methods tables found for 'rowMeans' 
4: multiple methods tables found for 'rowSums' 
> data(exampleMethyGenoSet)
> heatmapByChromosome(exampleMethyGenoSet, gene='6493',
+                     genomicFeature='TxDb.Hsapiens.UCSC.hg19.knownGene', includeGeneBody=TRUE)
Error: Expectation Failed

 

Because it's just copy and paste of example code, I don't figure out what causes this error.

Can anyone help me with this error?

Thanks,

Tom

--------------

> sessionInfo()
R version 3.3.0 (2016-05-03)
Platform: x86_64-apple-darwin13.4.0 (64-bit)
Running under: OS X 10.9.5 (Mavericks)

locale:
[1] C

attached base packages:
 [1] stats4    parallel  grid      stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
[1] methyAnalysis_1.14.0 org.Hs.eg.db_3.3.0   AnnotationDbi_1.34.2 Biobase_2.32.0       GenomicRanges_1.24.0
[6] GenomeInfoDb_1.8.1   IRanges_2.6.0        S4Vectors_0.10.0     BiocGenerics_0.18.0 

loaded via a namespace (and not attached):
 [1] nlme_3.1-128                  bitops_1.0-6                  matrixStats_0.50.2            RColorBrewer_1.1-2           
 [5] httr_1.1.0                    tools_3.3.0                   doRNG_1.6                     nor1mix_1.2-1                
 [9] R6_2.1.2                      affyio_1.42.0                 rpart_4.1-10                  KernSmooth_2.23-15           
[13] Hmisc_3.17-4                  DBI_0.4-1                     Gviz_1.16.1                   mgcv_1.8-12                  
[17] colorspace_1.2-6              nnet_7.3-12                   methylumi_2.18.2              gridExtra_2.2.1              
[21] base64_2.0                    preprocessCore_1.34.0         chron_2.3-47                  pkgmaker_0.22                
[25] rtracklayer_1.32.0            scales_0.4.0                  genefilter_1.54.2             quadprog_1.5-5               
[29] affy_1.50.0                   stringr_1.0.0                 digest_0.6.9                  Rsamtools_1.24.0             
[33] foreign_0.8-66                illuminaio_0.14.0             siggenes_1.46.0               GEOquery_2.38.4              
[37] lumi_2.24.0                   XVector_0.12.0                htmltools_0.3.5               dichromat_2.0-0              
[41] BSgenome_1.40.0               ensembldb_1.4.2               limma_3.28.4                  RSQLite_1.0.0                
[45] shiny_0.13.2                  BiocInstaller_1.22.2          mclust_5.2                    BiocParallel_1.6.2           
[49] acepack_1.3-3.3               VariantAnnotation_1.18.1      RCurl_1.95-4.8                magrittr_1.5                 
[53] Formula_1.2-1                 Matrix_1.2-6                  Rcpp_0.12.5                   munsell_0.4.3                
[57] stringi_1.0-1                 nleqslv_3.0.1                 MASS_7.3-45                   genoset_1.28.2               
[61] SummarizedExperiment_1.2.2    zlibbioc_1.18.0               AnnotationHub_2.4.2           plyr_1.8.3                   
[65] bumphunter_1.12.0             minfi_1.18.2                  lattice_0.20-33               Biostrings_2.40.0            
[69] splines_3.3.0                 multtest_2.28.0               GenomicFeatures_1.24.2        annotate_1.50.0              
[73] locfit_1.5-9.1                beanplot_1.2                  rngtools_1.2.4                codetools_0.2-14             
[77] biomaRt_2.28.0                XML_3.98-1.4                  biovizBase_1.20.0             latticeExtra_0.6-28          
[81] data.table_1.9.6              httpuv_1.3.3                  foreach_1.4.3                 gtable_0.2.0                 
[85] openssl_0.9.3                 reshape_0.8.5                 ggplot2_2.1.0                 mime_0.4                     
[89] xtable_1.8-2                  survival_2.39-4               iterators_1.0.8               GenomicAlignments_1.8.0      
[93] registry_0.3                  cluster_2.0.4                 interactiveDisplayBase_1.10.3
methyanalysis • 1.8k views
ADD COMMENT

Login before adding your answer.

Traffic: 525 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6