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Tom
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@tom-10763
Last seen 5.2 years ago
Hello,
I'm working on illumina methylation array data. Focusing on the methylation pattern at 11p15.5, a imprinted region, I plan to use "methyAnalysis" to draw a heatmap by chromosome location.
Before actually analysing my data, I first tried the example codes and got the following error:
> library(methyAnalysis)
Loading required package: grid
Loading required package: BiocGenerics
Loading required package: parallel
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:parallel':
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ, clusterExport,
clusterMap, parApply, parCapply, parLapply, parLapplyLB, parRapply,
parSapply, parSapplyLB
The following objects are masked from 'package:stats':
IQR, mad, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, append, as.data.frame,
cbind, colnames, do.call, duplicated, eval, evalq, get, grep, grepl,
intersect, is.unsorted, lapply, lengths, mapply, match, mget, order, paste,
pmax, pmax.int, pmin, pmin.int, rank, rbind, rownames, sapply, setdiff, sort,
table, tapply, union, unique, unsplit
Loading required package: IRanges
Loading required package: S4Vectors
Loading required package: stats4
Attaching package: 'S4Vectors'
The following objects are masked from 'package:base':
colMeans, colSums, expand.grid, rowMeans, rowSums
Loading required package: GenomeInfoDb
Loading required package: GenomicRanges
Loading required package: Biobase
Welcome to Bioconductor
Vignettes contain introductory material; view with 'browseVignettes()'. To
cite Bioconductor, see 'citation("Biobase")', and for packages
'citation("pkgname")'.
Loading required package: org.Hs.eg.db
Loading required package: AnnotationDbi
Setting options('download.file.method.GEOquery'='auto')
Setting options('GEOquery.inmemory.gpl'=FALSE)
No methods found in "GenomicRanges" for requests: overlapsAny
No methods found in "genoset" for requests: rowRanges, rowRanges<-, toGenomeOrder
Warning messages:
1: multiple methods tables found for 'colMeans'
2: multiple methods tables found for 'colSums'
3: multiple methods tables found for 'rowMeans'
4: multiple methods tables found for 'rowSums'
> data(exampleMethyGenoSet)
> heatmapByChromosome(exampleMethyGenoSet, gene='6493',
+ genomicFeature='TxDb.Hsapiens.UCSC.hg19.knownGene', includeGeneBody=TRUE)
Error: Expectation Failed
Because it's just copy and paste of example code, I don't figure out what causes this error.
Can anyone help me with this error?
Thanks,
Tom
--------------
> sessionInfo() R version 3.3.0 (2016-05-03) Platform: x86_64-apple-darwin13.4.0 (64-bit) Running under: OS X 10.9.5 (Mavericks) locale: [1] C attached base packages: [1] stats4 parallel grid stats graphics grDevices utils datasets methods base other attached packages: [1] methyAnalysis_1.14.0 org.Hs.eg.db_3.3.0 AnnotationDbi_1.34.2 Biobase_2.32.0 GenomicRanges_1.24.0 [6] GenomeInfoDb_1.8.1 IRanges_2.6.0 S4Vectors_0.10.0 BiocGenerics_0.18.0 loaded via a namespace (and not attached): [1] nlme_3.1-128 bitops_1.0-6 matrixStats_0.50.2 RColorBrewer_1.1-2 [5] httr_1.1.0 tools_3.3.0 doRNG_1.6 nor1mix_1.2-1 [9] R6_2.1.2 affyio_1.42.0 rpart_4.1-10 KernSmooth_2.23-15 [13] Hmisc_3.17-4 DBI_0.4-1 Gviz_1.16.1 mgcv_1.8-12 [17] colorspace_1.2-6 nnet_7.3-12 methylumi_2.18.2 gridExtra_2.2.1 [21] base64_2.0 preprocessCore_1.34.0 chron_2.3-47 pkgmaker_0.22 [25] rtracklayer_1.32.0 scales_0.4.0 genefilter_1.54.2 quadprog_1.5-5 [29] affy_1.50.0 stringr_1.0.0 digest_0.6.9 Rsamtools_1.24.0 [33] foreign_0.8-66 illuminaio_0.14.0 siggenes_1.46.0 GEOquery_2.38.4 [37] lumi_2.24.0 XVector_0.12.0 htmltools_0.3.5 dichromat_2.0-0 [41] BSgenome_1.40.0 ensembldb_1.4.2 limma_3.28.4 RSQLite_1.0.0 [45] shiny_0.13.2 BiocInstaller_1.22.2 mclust_5.2 BiocParallel_1.6.2 [49] acepack_1.3-3.3 VariantAnnotation_1.18.1 RCurl_1.95-4.8 magrittr_1.5 [53] Formula_1.2-1 Matrix_1.2-6 Rcpp_0.12.5 munsell_0.4.3 [57] stringi_1.0-1 nleqslv_3.0.1 MASS_7.3-45 genoset_1.28.2 [61] SummarizedExperiment_1.2.2 zlibbioc_1.18.0 AnnotationHub_2.4.2 plyr_1.8.3 [65] bumphunter_1.12.0 minfi_1.18.2 lattice_0.20-33 Biostrings_2.40.0 [69] splines_3.3.0 multtest_2.28.0 GenomicFeatures_1.24.2 annotate_1.50.0 [73] locfit_1.5-9.1 beanplot_1.2 rngtools_1.2.4 codetools_0.2-14 [77] biomaRt_2.28.0 XML_3.98-1.4 biovizBase_1.20.0 latticeExtra_0.6-28 [81] data.table_1.9.6 httpuv_1.3.3 foreach_1.4.3 gtable_0.2.0 [85] openssl_0.9.3 reshape_0.8.5 ggplot2_2.1.0 mime_0.4 [89] xtable_1.8-2 survival_2.39-4 iterators_1.0.8 GenomicAlignments_1.8.0 [93] registry_0.3 cluster_2.0.4 interactiveDisplayBase_1.10.3