Hi,

In the FEM vignette it's written (page 7) : https://www.bioconductor.org/packages/release/bioc/html/FEM.html

" For each gene g in the maximally connected subnetwork, we then derive a statistic of association between its DNA methylation profile and the phenotype of interest (POI) (here normal/cancer status), denoted by t(D,g) as well as between its mRNA expression pro le and the same POI, which we denote by t(R,g). These statistics have already been computed beforehand using the limma package. "

Can someone explain me how to do that using limma ?

So I've two matrix (one containing the methylation state of each gene and samples, one containing the expression of each gene and samples).

Thank you