Hi I was wondering if anyone has any opinions about the SPOT spotfinding software from csiro (I.e the package that runs in R) and it's limitations, benefits, opinion about batch performance accuracy etc. Has anyone done any direct comparisons of SPOT with Genepix or other spotfinding software avalible ? Or is anyone routinely using SPOT and highly recommend it... I'm currently comparing it to genepix data from a relatively old version of genepix with kooperberg background subtraction and using limma for all subsequent analysis. many thanks Jason -- -------------------------------- Jason Skelton Pathogen Microarrays Wellcome Trust Sanger Institute Hinxton Cambridge CB10 1SA Tel +44(0)1223 834244 Ext 7123 Fax +44(0)1223 494919