Hi all,
I've just started using flowsom and ive run into a few issues:
1. flowsom takes direct input of the fcs files but wont take a flowset directly
fs<-read.flowSet(jfiles.out)
> fs
A flowSet with 8 experiments.
fs=ReadInput(fset)
Error in ReadInput(fset) : Inputs of type list are not supported.
Please supply either a FlowFrame, a FlowSet or an array
of valid paths to files or directories.
However I've been able to use readinput directly:
> fs=ReadInput(jfiles.out)
Reading file TCGA-05-4244-01Z-00-DX1_19500_19500_500_500_LUAD/TCGA-05-4244-01Z-00-DX1_19500_19500_500_500_LUAD_jun_human.fcs ....
2. I get an error message when using metaclustering_consensus
the following work:
fs2=BuildSOM(fs)
fs3<-BuildMST(fs2)
then i try:
fs4=metaClustering_consensus(fs3,k=10)
Error in ConsensusClusterPlus::ConsensusClusterPlus(t(data), maxK = k, :
d must be a matrix, distance object or ExpressionSet (eset object)
Thanks for your help!
jeremy