Hello all,

I am trying to use geNorm in R to get some housekeeping genes selected. But I can't seem to get read.qPCR to play nice. I am getting the error: "Error in levels(raw.data$Sample) <- make.names(levels(raw.data$Sample)) : 
  attempt to set an attribute on NULL"

I've tried to debug it but haven't made any progress.

Using the code:

## Load packages and read in data

rm(list = ls())

library(ReadqPCR)

library(NormqPCR)

library(RColorBrewer)

setwd("...")

data <- read.qPCR(file.path(getwd(), "HK_test_2.csv"), verbose = TRUE)

> sessionInfo()
R version 3.3.1 (2016-06-21)
Platform: x86_64-apple-darwin13.4.0 (64-bit)
Running under: OS X 10.11.3 (El Capitan)

locale:
[1] en_GB.UTF-8/en_GB.UTF-8/en_GB.UTF-8/C/en_GB.UTF-8/en_GB.UTF-8

attached base packages:
[1] parallel  stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] NormqPCR_1.18.0     qpcR_1.4-0          Matrix_1.2-6        robustbase_0.92-6  
 [5] rgl_0.95.1441       minpack.lm_1.2-0    MASS_7.3-45         RColorBrewer_1.1-2 
 [9] ReadqPCR_1.18.0     affy_1.50.0         Biobase_2.32.0      BiocGenerics_0.18.0

loaded via a namespace (and not attached):
[1] lattice_0.20-33       grid_3.3.1            BiocInstaller_1.22.3 
[4] zlibbioc_1.18.0       affyio_1.42.0         preprocessCore_1.34.0
[7] tools_3.3.1           DEoptimR_1.0-6       

Here is my data:

I would really appreciate any advice as to how to remedy this?

Thanks,

Tim