How to combine replicates creating heatmap?
1
0
Entering edit mode
@alexandrgopanenko-11598
Last seen 17 months ago
Germany

Hello!

When I do DE analysis of my RNA-seq data I often have 3-4 replicates of each condition. So, I want present heatmap contaning only condition1_vs_condition_2.

I realize that it will not contain information about variations between replicates but I think it will be easy for understanding.

 

Thank you in advance,

Alexander Gopanenko

deseq2 edgeR heatmap.2 • 2.6k views
ADD COMMENT
0
Entering edit mode

By replicates are these technical or biological replicates? I would never combine biological replicates in a heatmap, especially as you have only 2 conditions I can't see the point in that.

ADD REPLY
1
Entering edit mode
@mikelove
Last seen 1 day ago
United States

A heatmap is not a good way to show the differences between two numbers. Humans are not good at judging values by color. Plots showing spatial distances are much better.

Previous post on the topic:

A: DESeq heatmap based on threshold

ADD COMMENT
0
Entering edit mode

Michael, thank you very much for your advice!

ADD REPLY

Login before adding your answer.

Traffic: 718 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6