Entering edit mode
Question is in the title.
I know there is a collapseReplicates function in DESEQ2. But I didn't succeed to use it so I did the sum up manually.
In the vignette ,
The purpose of this function is to sum up read counts from technical replicates to create an object with a single column of read counts for each sample
It doesn't say that they divide after sum up...I was wondering if i need to divide the raw counts by the number of technical replicates added because it will have an impact on the size factor ?
Thanks