read.idat and empty Symbols
1
0
Entering edit mode
h.mon • 0
@hmon-8976
Last seen 4.1 years ago
Brazil

I am reading Illumina Human HT-12 v4 Expression BeadChip with read.idat from the limma package. While the reading works apparently without problems, the resulting object has lots (3270, to be exact) of empty strings for genes$Symbols.

What may be causing this?

> idatfiles <- list.files( path = "../array", pattern = ".idat$", full.names = TRUE )
> bgxfile <- list.files( path = "../array", pattern = ".bgx$", full.names = TRUE )
> x <- read.idat( idatfiles, bgxfile, dateinfo = T )

> length( which( y$genes$Symbol == "", arr.ind = F ) )
[1] 3270
> y$genes[8446,]
         Probe_Id Array_Address_Id Symbol
8446 ILMN_1906423          5310327    

And here is one example of the correnponding annotation from the bgx file:

Homo sapiens    Unigene    Hs.390407    ILMN_89369    HS.390407    Hs.390407        Hs.390407        27828963    BX097705            ILMN_1906423    0005310327    S    640    GAGAGGCAGGGTGAAGAGGTCGAAGGAGCCTGAGTTAGCAGGGATGAGCA    2    -    87520225-87520274        BX097705 NCI_CGAP_Kid5 Homo sapiens cDNA clone IMAGp998E053890, mRNA sequence                    

 

> sessionInfo()

R version 3.3.2 (2016-10-31)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Debian GNU/Linux stretch/sid

locale:
 [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C               LC_TIME=en_US.UTF-8       
 [4] LC_COLLATE=en_US.UTF-8     LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
 [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                  LC_ADDRESS=C              
[10] LC_TELEPHONE=C             LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       

attached base packages:
[1] parallel  stats4    stats     graphics  grDevices utils     datasets  methods  
[9] base     

other attached packages:
 [1] GO.db_3.3.0                SummarizedExperiment_1.2.3 GenomicRanges_1.24.3      
 [4] GenomeInfoDb_1.8.7         RColorBrewer_1.1-2         pheatmap_1.0.8            
 [7] ggplot2_2.2.0              pathview_1.12.0            gage_2.22.0               
[10] org.Hs.eg.db_3.3.0         AnnotationDbi_1.34.4       IRanges_2.6.1             
[13] S4Vectors_0.10.3           Biobase_2.32.0             BiocGenerics_0.18.0       
[16] illuminaio_0.14.0          limma_3.28.21             

loaded via a namespace (and not attached):
 [1] Rcpp_0.12.8        plyr_1.8.4         XVector_0.12.1     tools_3.3.2       
 [5] zlibbioc_1.18.0    digest_0.6.10      base64_2.0         RSQLite_1.1       
 [9] memoise_1.0.0      tibble_1.2         gtable_0.2.0       png_0.1-7         
[13] KEGGgraph_1.30.0   graph_1.50.0       DBI_0.5-1          Rgraphviz_2.16.0  
[17] curl_2.3           httr_1.2.1         Biostrings_2.40.2  grid_3.3.2        
[21] R6_2.2.0           XML_3.98-1.5       org.Bt.eg.db_3.3.0 scales_0.4.1      
[25] KEGGREST_1.12.3    assertthat_0.1     colorspace_1.3-1   openssl_0.9.5     
[29] lazyeval_0.2.0     munsell_0.4.3    

illuminaio limma • 1.2k views
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1
Entering edit mode
@james-w-macdonald-5106
Last seen 7 hours ago
United States

Not everything has a HUGO symbol - those are reserved for transcripts that are considered to be an actual thing. In other words, the example you have put forth is an IMAGE clone, which is at present a hypothetical transcript that may or may not get transcribed in humans. This particular clone was uploaded to GenBank in 2003 and hasn't really been updated since, so I would bet nobody has ever detected it in the wild, so it just persists as a hypothetical in NCBI's databases.

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Entering edit mode

Thanks. I will investigate some other probes, but now I am less anxious abut the subject.

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