Error in qAlign, QuasR, in paired-end RNA Seq
0
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@guillaumedachy-11994
Last seen 7 months ago
Brussels

Hello !

I'm trying to use QuasR but I have a new error message using qAlign :

The early beginning goes well, and then I get this : (I use as genome "BSgenome.Hsapiens.UCSC.hg19" and a auxiliary genome)

Available cores:
nodeNames
ae1.jbd
      1
Performing genomic alignments for 2 samples. See progress in the log file:
/home/ahmed/QuasR_log_7a8251cb7337.txt
[fai_load] build FASTA index.
[fai_build_core] no entries in file
[fai_load] fail to open FASTA index.
Error in checkForRemoteErrors(val) :
  one node produced an error: Error on ae1.jbd processing sample /tmp/RtmpEoXA7z/RC_FA225_TTAGGC_R1.fastq.gz1c5d69c7fbef.fastq : error in evaluating the argument 'file' in selecting a method for function 'scanFaIndex': Error in value[[3L]](cond) : 'open' index failed
  file: /tmp/RtmpEoXA7z/file1c5d3e2b67b4.fa
Calls: open ... tryCatch -> tryCatchList -> tryCatchOne -> <Anonymous>

 

Would someone have an explanation and advices to help me ?

Thank you so much !

 

Guillaume

quasr paired-end reads rnaseq • 481 views
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