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Hi all, I am trying to visualize mapped reads from nanopore sequencing by Gviz. Unluckily, even if I am able to visualize short reads from illumina alignments that we have, Gviz seems to fail to show long reads alignment from nanopore.
Is there any option I should use in order to use a bam obtained from long read sequencing?
here my code:
library(Gviz)
library(BSgenome.Hsapiens.UCSC.hg19)
afrom <- 44905200
ato <- 44947200
bam <- "path/to/my/sorted_indexed_bam.bam"
alTrack <- AlignmentsTrack(bam, isPaired = F)
sTrack <- SequenceTrack(Hsapiens)
plotTracks(c(alTrack, sTrack), chromosome = "chr21", from = afrom, to = ato)
R version 3.6.1 (2019-07-05)
Platform: x86_64-conda_cos6-linux-gnu (64-bit)
Matrix products: default
BLAS/LAPACK: /home/gianlucam/tools/anaconda2/envs/R/lib/R/lib/libRblas.so
locale:
[1] en_US.UTF-8
attached base packages:
[1] grid stats4 parallel stats graphics grDevices utils datasets methods base
other attached packages:
[1] Gviz_1.28.3 BSgenome.Hsapiens.UCSC.hg19_1.4.0 BSgenome_1.52.0 rtracklayer_1.44.4 Biostrings_2.52.0 XVector_0.24.0
[7] GenomicRanges_1.36.1 GenomeInfoDb_1.20.0 IRanges_2.18.3 S4Vectors_0.22.1 BiocGenerics_0.30.0
loaded via a namespace (and not attached):
[1] ProtGenerics_1.16.0 bitops_1.0-6 matrixStats_0.58.0 bit64_4.0.5 RColorBrewer_1.1-2 progress_1.2.2 httr_1.4.2 tools_3.6.1
[9] backports_1.2.1 utf8_1.2.1 R6_2.5.0 rpart_4.1-15 Hmisc_4.5-0 DBI_1.1.1 lazyeval_0.2.2 colorspace_2.0-0
[17] nnet_7.3-15 tidyselect_1.1.0 gridExtra_2.3 prettyunits_1.1.1 curl_4.3 bit_4.0.4 compiler_3.6.1 Biobase_2.44.0
[25] htmlTable_2.1.0 DelayedArray_0.10.0 scales_1.1.1 checkmate_2.0.0 stringr_1.4.0 digest_0.6.27 Rsamtools_2.0.3 foreign_0.8-76
[33] base64enc_0.1-3 dichromat_2.0-0 jpeg_0.1-8.1 pkgconfig_2.0.3 htmltools_0.5.1.1 ensembldb_2.8.1 fastmap_1.1.0 htmlwidgets_1.5.3
[41] rlang_0.4.10 rstudioapi_0.13 RSQLite_2.2.5 generics_0.1.0 BiocParallel_1.18.1 dplyr_1.0.5 VariantAnnotation_1.30.1 RCurl_1.98-1.3
[49] magrittr_2.0.1 GenomeInfoDbData_1.2.1 Formula_1.2-4 Matrix_1.3-2 Rcpp_1.0.6 munsell_0.5.0 fansi_0.4.2 lifecycle_1.0.0
[57] stringi_1.5.3 SummarizedExperiment_1.14.1 zlibbioc_1.30.0 blob_1.2.1 crayon_1.4.1 lattice_0.20-41 splines_3.6.1 GenomicFeatures_1.36.4
[65] hms_1.0.0 knitr_1.31 pillar_1.5.1 biomaRt_2.40.5 XML_3.99-0.3 glue_1.4.2 biovizBase_1.32.0 latticeExtra_0.6-29
[73] data.table_1.14.0 png_0.1-7 vctrs_0.3.7 gtable_0.3.0 purrr_0.3.4 assertthat_0.2.1 cachem_1.0.4 ggplot2_3.3.3
[81] xfun_0.22 AnnotationFilter_1.8.0 survival_3.2-10 tibble_3.1.0 GenomicAlignments_1.20.1 AnnotationDbi_1.46.1 memoise_2.0.0 cluster_2.1.1
[89] ellipsis_0.3.1