Hi I am new to analysis. I am trying to do some ATAC analysis. so I was reading through and wanted to understand the file output here is snap shot of the tutorial from http://bioconductor.org/packages/release/bioc/vignettes/ChIPseeker/inst/doc/ChIPseeker.html seqnames ranges strand | V4 V5

<Rle> <IRanges> <Rle> | <character> <numeric>

[1] chr1 815093-817883 * | MACS_peak_1 295.76

[2] chr1 1243288-1244338 * | MACS_peak_2 63.19

[3] chr1 2979977-2981228 * | MACS_peak_3 100.16

[4] chr1 3566182-3567876 * | MACS_peak_4 558.89

[5] chr1 3816546-3818111 * | MACS_peak_5 57.57

... ... ... ... . ... ...

[1327] chrX 135244783-135245821 * | MACS_peak_1327 55.54

[1328] chrX 139171964-139173506 * | MACS_peak_1328 270.19

[1329] chrX 139583954-139586126 * | MACS_peak_1329 918.73

[1330] chrX 139592002-139593238 * | MACS_peak_1330 210.88

[1331] chrY 13845134-13845777 * | MACS_peak_1331 58.39

-------

could you tell me why is there a * under the strand column instead of + / -

With ATAC-Seq you are detecting regions of accessible DNA, which isn't strand specific, so you shouldn't expect a peak to be on a particular strand.