I have a couple of questions about DESeq2.
1) Could we use it for repeated measured outcome? Do we only need to add a time variable in the design as below?
dds <- DESeqDataSetFromMatrix(countData = cts, colData = coldata, design= ~ time + condition)
2) For the condition variable, could we use continuous outcome here? Or we can only use the factor variable for condition due to NB distribution behind the DESeq2?
3) I saw you mentioned that DESeq2 only works for replicates data, here replicates data means biological replicate or technical replicate?
Thanks in advance.