Dear all,
I have come across examples of people filtering out immunoglobulin genes during RNA-seq analysis, such as in this paper :
https://www.nature.com/articles/s41467-019-11373-9
However, I am interested in the immunology of the condition I am studying and hence in the expression of these genes.
Is there a reason why they should be removed?
If yes should these genes, alongside non-coding genes, pseudogenes etc. be filtered before or after normalisation & differential expression analysis with Deseq?
Thank you for the thoughts.
Hi Gordon Smyth , I read your paper with great interests! I've got one basic question regarding Ig gene segments- I want to know your thoughts on Ig gene segments as "not part of the regular transcriptome" I've seen some papers that still consider them as normal gene expression, I would really appreciate your comment!
Thanks,