Read Cellranger outputs in R, combine as SingleCellExperiment
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@d9ae38d8
Last seen 3 months ago
Belgium

Hi !

I have Cellranger -multi outputs from a 10x 5' GEX + Antibody barcodes + VDJ pipeline.

I wish to analyse my data in R and so to create a SingleCellExperiment Object (sce) that encompasses all the data.

Using the DropletUtils::read10xCounts() function I was able to create such an object for Gene expression and Antibody barcodes. However I cannot add a slot for VDJ data, which are not included in the feature_bc_matrix.h5 matrix.

Is there any well-defined way, package, to create a full data sce object that encompasses all three libraries info?

Thanks !

ReadInR Cellranger GEX+Features+VDJ • 137 views
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