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Hi everyone,
I have two HiC data from two different conditions. I would like to calculate the differential interaction between these two conditions using HiCDCplus package. Is it possible to run hicdiff without replicates?
if yes, how?
if no, is there any other way to do so from .hic files?
Thanks much
Best,
TA
hicdcdiffrelies onDESeq2, which doesn't allow comparison of two conditions without replicates as significances cannot be determined reliably. You can check https://www.biostars.org/p/398152/ for a comprehensive set of discussions and links relating to this matter.