Not obtaining significant Gene Ontology Biological Process hits
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Jordan • 0
@516457fa
Last seen 23 months ago
United States

Hello, I'm having trouble obtaining significant GO hits using my protein list as universe. Regardless of the p value cutoff, I'm unable to obtain hits for my differentially expressed protein list due to the adjusted p value for all of the GO terms being above 0.05. For this analysis, I'm using the enrichGO function as part of the clusterprofiler package in R. I have also tried using external websites that perform GO analysis as well other adjusted methods but still do not obtain any GO hits. Is it simply that no hits are found in DEP using the protein list as universe? I provide my protein list (universe) and differentially expressed protein list below as well as the enrichGO function and output. Thanks

clusterProfiler • 610 views
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