DESeq2 analysis has not been run for this contrast
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Wang • 0
@e16bdcce
Last seen 2.4 years ago
Hong Kong

error :DESeq2 analysis has not been run for this contrast

DB.DESeq2 = 0

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I think until this step everything is ok enter image description here

here is my file↓

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here is my code ↓


# dbObj_contrast <- dba.contrast(dbObj_count, contrast=c("Condition","Responsive","Resistant"))
#dbObj_contrast 
#dbObj_analyze <- dba.analyze(dbObj_contrast)
#dba.show(dbObj_analyze,bContrasts=TRUE)
R DiffBind • 1.3k views
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ATpoint ★ 4.6k
@atpoint-13662
Last seen 1 day ago
Germany

The warnings come from the ashr shrinkage: Warnings when using "ashr" in lfcshrinkage for DESeq2

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After I install the package "stephenslab/mixsqp" I still have the same problem.

My DB.DESeq2 is shows 0.

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