Error with tximeta
1
0
Entering edit mode
jtobias ▴ 10
@jtobias-22448
Last seen 14 months ago
United States

Enter the body of text here

Code should be placed in three backticks as shown below


se <- tximeta(coldata)
Error in parse_con(txt, bigint_as_char) : 
  lexical error: invalid char in json text.
          d": 0,     "percent_mapped": NaN,     "call": "quant",     "
                     (right here) ------^
sessionInfo( )

R version 4.2.1 (2022-06-23)
Platform: x86_64-apple-darwin17.0 (64-bit)
Running under: macOS Monterey 12.5.1

Matrix products: default
LAPACK: /Library/Frameworks/R.framework/Versions/4.2/Resources/lib/libRlapack.dylib

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages:
[1] stats4    stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] PCAtools_2.8.0              ggrepel_0.9.1               DESeq2_1.36.0               org.Hs.eg.db_3.15.0         org.Mm.eg.db_3.15.0         AnnotationDbi_1.58.0       
 [7] forcats_0.5.2               stringr_1.4.1               dplyr_1.0.9                 purrr_0.3.4                 tidyr_1.2.0                 tibble_3.1.8               
[13] ggplot2_3.3.6               tidyverse_1.3.2             SummarizedExperiment_1.26.1 Biobase_2.56.0              GenomicRanges_1.48.0        GenomeInfoDb_1.32.3        
[19] IRanges_2.30.1              S4Vectors_0.34.0            BiocGenerics_0.42.0         MatrixGenerics_1.8.1        matrixStats_0.62.0          biomaRt_2.52.0             
[25] tximeta_1.14.1              readr_2.1.2                

loaded via a namespace (and not attached):
  [1] readxl_1.4.1                  backports_1.4.1               AnnotationHub_3.4.0           BiocFileCache_2.4.0           plyr_1.8.7                    lazyeval_0.2.2               
  [7] splines_4.2.1                 BiocParallel_1.30.3           digest_0.6.29                 ensembldb_2.20.2              htmltools_0.5.3               fansi_1.0.3                  
 [13] magrittr_2.0.3                memoise_2.0.1                 ScaledMatrix_1.4.0            googlesheets4_1.0.1           tzdb_0.3.0                    Biostrings_2.64.1            
 [19] annotate_1.74.0               modelr_0.1.9                  prettyunits_1.1.1             colorspace_2.0-3              blob_1.2.3                    rvest_1.0.3                  
 [25] rappdirs_0.3.3                haven_2.5.1                   crayon_1.5.1                  RCurl_1.98-1.8                jsonlite_1.8.0                tximport_1.24.0              
 [31] genefilter_1.78.0             survival_3.4-0                glue_1.6.2                    gtable_0.3.0                  gargle_1.2.0                  zlibbioc_1.42.0              
 [37] XVector_0.36.0                DelayedArray_0.22.0           BiocSingular_1.12.0           scales_1.2.1                  DBI_1.1.3                     Rcpp_1.0.9                   
 [43] xtable_1.8-4                  progress_1.2.2                dqrng_0.3.0                   bit_4.0.4                     rsvd_1.0.5                    httr_1.4.4                   
 [49] RColorBrewer_1.1-3            ellipsis_0.3.2                pkgconfig_2.0.3               XML_3.99-0.10                 dbplyr_2.2.1                  locfit_1.5-9.6               
 [55] utf8_1.2.2                    reshape2_1.4.4                tidyselect_1.1.2              rlang_1.0.4                   later_1.3.0                   munsell_0.5.0                
 [61] BiocVersion_3.15.2            cellranger_1.1.0              tools_4.2.1                   cachem_1.0.6                  cli_3.3.0                     generics_0.1.3               
 [67] RSQLite_2.2.16                broom_1.0.0                   fastmap_1.1.0                 yaml_2.3.5                    bit64_4.0.5                   fs_1.5.2                     
 [73] KEGGREST_1.36.3               AnnotationFilter_1.20.0       sparseMatrixStats_1.8.0       mime_0.12                     xml2_1.3.3                    compiler_4.2.1               
 [79] rstudioapi_0.13               filelock_1.0.2                curl_4.3.2                    png_0.1-7                     interactiveDisplayBase_1.34.0 reprex_2.0.2                 
 [85] geneplotter_1.74.0            stringi_1.7.8                 GenomicFeatures_1.48.3        lattice_0.20-45               ProtGenerics_1.28.0           Matrix_1.4-1                 
 [91] vctrs_0.4.1                   pillar_1.8.1                  lifecycle_1.0.1               BiocManager_1.30.18           cowplot_1.1.1                 bitops_1.0-7                 
 [97] irlba_2.3.5                   httpuv_1.6.5                  rtracklayer_1.56.1            R6_2.5.1                      BiocIO_1.6.0                  promises_1.2.0.1             
[103] codetools_0.2-18              assertthat_0.2.1              rjson_0.2.21                  withr_2.5.0                   GenomicAlignments_1.32.1      Rsamtools_2.12.0             
[109] GenomeInfoDbData_1.2.8        parallel_4.2.1                hms_1.1.2                     grid_4.2.1                    beachmat_2.12.0               DelayedMatrixStats_1.18.0    
[115] googledrive_2.0.0             shiny_1.7.2                   lubridate_1.8.0               restfulr_0.0.15
tximeta • 751 views
ADD COMMENT
1
Entering edit mode
jtobias ▴ 10
@jtobias-22448
Last seen 14 months ago
United States

I found the problem, and it's not with tximeta. Despite the fastq files existing (but being empty), salmon produced a quant.sf file, and wrote into ../aux_info/meta_info.json ""percent_mapped": NaN,"

I didn't know there was an issue with that sample until tximeta couldn't parse meta_info.json.

It might have been helpful for tximeta to report which .json file had the unusable information.

ADD COMMENT
0
Entering edit mode

The error is coming from jsonlite, upon trying to read a JSON file, called by tximport. The idea I had when writing this function was that it tells you the file number that hit a failure, because it counts off from 1 to #files. Did that not happen?

ADD REPLY

Login before adding your answer.

Traffic: 520 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6