Hello All,
We hope to collect some data from specially tagged mouse cells with lacZ. Other than the fact that they are tagged with lacZ, they don't have a distinct marker profile, by which these cells can be recognized.
So, my question is- If we do single cell on these samples, how can we recognize lacZ expressing cells (after mapping to the mouse genome?) Is it even possible?
Or if it isn't, is there a different tagging strategy we need to use to allow us to do a single cell study?
Any comments, suggestions, or pointers to literature, will be greatly appreciated
Thanks, Kavi
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