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Showing :
SingleCell
•
reset
3
votes
1
reply
371
views
'Seurat' package
singlecell
seurat
updated 4 months ago by
ATpoint
★ 3.4k • written 4 months ago by
Sooni
▴ 10
0
votes
1
reply
299
views
Choosing the right lower limit for emptyDrops
SingleCell
emptyDrops
10X
updated 4 months ago by
Peter Hickey
▴ 650 • written 4 months ago by
sandra.garces.19
• 0
0
votes
2
replies
299
views
Why my log2fcs are too high?
DESeq2
SingleCell
log2FC
5 months ago
paria.alipour
• 0
0
votes
2
replies
318
views
Scaling and normalization by deconvolution of SMART-Seq 2 data
scran
Clustering
SingleCell
Normalization
6 months ago
Assa Yeroslaviz
★ 1.5k
1
vote
4
replies
377
views
Single-cell differential expression analysis with multiple samples
SingleCell
6 months ago
iaradsouza1
• 0
0
votes
7
replies
630
views
fail to annotate chromosome data to single cell object
error
SingleCell
annotate
6 months ago
sandra.garces.19
• 0
0
votes
0
replies
290
views
Job:
Open position in the CBIO lab: single-cell proteomics
Proteomics
SingleCell
JobPosting
MassSpectrometry
6 months ago
Laurent Gatto
1.6k
0
votes
3
replies
588
views
Knee and Inflection of DropletUtils
SingleCell
updated 6 months ago by
ATpoint
★ 3.4k • written 7 months ago by
ankuuu
• 0
0
votes
1
reply
1.0k
views
DESeq2 after Seurat analysis on integrated datasets
DESeq2
SingleCell
GeneExpression
Seurat
updated 7 months ago by
ATpoint
★ 3.4k • written 7 months ago by
bio.boi
• 0
1
vote
1
reply
355
views
Pseudobulk running all groups together vs subsetting first
DESeq2
edgeR
SingleCell
updated 7 months ago by
Wolfgang Huber
13k • written 7 months ago by
nhaus
• 0
0
votes
1
reply
316
views
Job:
Reopened: 'Omics data analyst for HPCBio, University of Illinois
SingleCell
Spatial
RNA-seq
7 months ago
Jenny Drnevich
★ 2.0k
0
votes
3
replies
533
views
Tissue Level Differential Expression Analysis
DESeq2
DifferentialExpression
differentialex
Pseudobulk
SingleCell
updated 9 months ago by
Michael Love
40k • written 9 months ago by
skanda.hebbale
• 0
0
votes
1
reply
386
views
Job:
'Omics data analyst for HPCBio, University of Illinois
SingleCell
rnaseq
methylationArrayAnalysis
Spatial
written 9 months ago by
Jenny Drnevich
★ 2.0k
0
votes
1
reply
492
views
Single-Cell Data Simulation from Bulk RNA-seq
SingleCell
RNASeq
RNASeqData
scRNAseq
updated 10 months ago by
Gordon Smyth
48k • written 10 months ago by
irvin.ng
• 0
0
votes
1
reply
537
views
allele count data bioc object
SingleCell
SingleCellMultiModal
allele
updated 11 months ago by
Michael Love
40k • written 11 months ago by
oliver.crook
• 0
0
votes
0
replies
528
views
Job:
Postdoctoral Fellow in Epigenomcis/Neurobiology (MGH/Harvard Medical School, Boston)
GenomicVariation
SingleCell
Autism
Neurogenesis
Epigenetics
11 months ago
sudhir.thakurela
• 0
0
votes
0
replies
340
views
Gene Id duplicated
SingleCell
SingleCellData
updated 12 months ago by
ATpoint
★ 3.4k • written 12 months ago by
Romina
• 0
0
votes
1
reply
423
views
Set up "mock" cell type as the reference?
DESeq2
SingleCell
updated 12 months ago by
Michael Love
40k • written 12 months ago by
Yiqi
▴ 10
0
votes
0
replies
275
views
weighting sample group
monocle
SingleCell
DifferentialExpression
12 months ago
Sogand
• 0
2
votes
2
replies
476
views
How to detect LacZ tagged cells from single cell
SingleCell
SingleCellExperiment
SingleCellWorkflow
SingleCellData
12 months ago
k1m
▴ 20
0
votes
2
replies
551
views
how to plot a fitting line on the testPseudotime
Pseudotime
testPseudotime
TSCAN
SingleCell
OSCA
12 months ago
Guandong Shang
▴ 40
2
votes
4
replies
616
views
Summary of library size factor is all equal to one
SingleCell
SingleCellData
SingleCellWorkflow
SingleCellExperiment
12 months ago
Romina
• 0
0
votes
1
reply
791
views
Error in .testForValidKeys(x, keys, keytype, fks) :
SingleCell
AnnotationDbi
updated 13 months ago by
James W. MacDonald
63k • written 13 months ago by
techgirl2022
• 0
0
votes
1
reply
597
views
Error in running: classifySingleR from SingleR package
SingleR
SingleCell
updated 14 months ago by
ATpoint
★ 3.4k • written 14 months ago by
amit.korngut
• 0
1
vote
2
replies
842
views
DESeq2 aggregated single cell data
pseudobulk
SingleCell
DESeq2
14 months ago
fluentin44
• 0
0
votes
0
replies
428
views
Differential expression with single cell data: any good two component (hurdle) models recently?
SingleCellWorkflow
DifferentialExpression
SingleCell
SingleCellMultiModal
MAST
15 months ago
Nik Tuzov
▴ 80
0
votes
0
replies
485
views
Performing DGE analysis with MAST on a variable with more than 2 levels
MAST
DEG
SingleCell
16 months ago
zroger499
• 0
2
votes
1
reply
505
views
() followed by [] within an argument in singlecell OSCA workflow
SingleCell
R
OSCA
updated 17 months ago by
jeroen.gilis
▴ 90 • written 17 months ago by
BioInfoBeginnerrr
• 0
2
votes
1
reply
1.2k
views
Convert AnnotatedDataFrame S4 object from GEO datasets to Seurat Object
biobase
SingleCellData
seurat
SingleCell
updated 20 months ago by
James W. MacDonald
63k • written 20 months ago by
NGS_enthusiast
• 0
0
votes
0
replies
509
views
Issues geting meaninfull results for single cell DGE using MAST
MAST
SingleCell
20 months ago
zroger499
• 0
0
votes
0
replies
676
views
Job:
Postdoc Opening in Bioinformatics or Computational Biology
SpatialData
scRNAseq
SingleCell
Omixer
20 months ago
Sebastian Lewandowski
• 0
2
votes
2
replies
900
views
velociraptor preparation
SingleCellExperiment
singlecell
velociraptor
scvelo
updated 21 months ago by
ATpoint
★ 3.4k • written 21 months ago by
Pietro
▴ 40
0
votes
0
replies
529
views
Job:
Research Scientists/Postdoctoral Candidates/Computational Biologists and Programmers excited about computational challenges in modeling CRISPR genome…
CRISPR
SingleCell
Genomics
24 months ago
lpinello
• 0
1
vote
2
replies
774
views
Using limma duplicateCorrelation to account for donor/dataset effects in scRNA-Seq pseudobulk counts from multiple sources
limma
SingleCell
24 months ago
Andrew Pattison
• 0
0
votes
0
replies
520
views
Spike-In Cells for Normalization?
SpikeIn
SingleCell
2.1 years ago
mb1996
• 0
0
votes
0
replies
503
views
Job:
Computational postdoctoral fellow for cancer disparities research (Boston University)
DNASeq
RNASeq
SingleCell
2.1 years ago
camp
• 0
0
votes
0
replies
642
views
Job:
Senior Bioinformatician - University of Alabama at Birmingham
JobPosting
Transcriptomics
SingleCell
RNA-seq
2.2 years ago
lianov
• 0
0
votes
1
reply
677
views
Differentially expressed genes across three different conditions
MAST
SingleCell
diffGeneAnalysis
updated 2.2 years ago by
James W. MacDonald
63k • written 2.2 years ago by
sofia.puvogel
• 0
3
votes
2
replies
1.6k
views
Merging only those samples sequenced over multiple batches - scRNA-seq
SingleCell
SingleCellExperiment
scater
2.6 years ago
camerond
• 0
0
votes
0
replies
716
views
Job:
Computational Biologist at Denali Therapeutics
Transcriptomics
SingleCell
JobPosting
2.7 years ago
Steve Lianoglou
13k
0
votes
2
replies
1.1k
views
Removal of cell cycle effect using batchelor::regressBatches() and proper usage of corrected UMI matrix
scRNAseq
batchelor
SingleCell
SingleCellExperiment
scran
2.8 years ago
jirkanov
• 0
2
votes
1
reply
1.5k
views
DESeq2 DE gene expression of scRNA-seq pseudobulk
DESeq2
SingleCell
updated 2.8 years ago by
Michael Love
40k • written 2.8 years ago by
Christian
• 0
0
votes
1
reply
637
views
improving running time of runNMF
runNMF
scater
singlecell
SingleCellExperiment
updated 2.9 years ago by
Aaron Lun
★ 28k • written 2.9 years ago by
lirongrossmann
▴ 40
0
votes
0
replies
563
views
Creating a stable batch-corrected scRNA-Seq data with multiple datasets and multiple integrations
scater
singlecell
scran
BatchEffect
2.9 years ago
hamza_karakurt
▴ 50
2
votes
6
replies
1.7k
views
Discarding ambient RNA using estimateAmbience
emptyDrop
ambient
singlecell
estimateAmbience
updated 3.0 years ago by
Aaron Lun
★ 28k • written 3.0 years ago by
lirongrossmann
▴ 40
0
votes
6
replies
1.9k
views
Discrepancy between cell type and cluster using SingleCellExperiment and SingleR
singlecell
singlecellexperiment
umap
singleR
updated 3.1 years ago by
Aaron Lun
★ 28k • written 3.1 years ago by
lirongrossmann
▴ 80
0
votes
2
replies
1.1k
views
most correlated genes in SingleR
singlecell
singleR
updated 3.1 years ago by
Aaron Lun
★ 28k • written 3.1 years ago by
lirongrossmann
▴ 40
0
votes
1
reply
1000
views
predicting cell type of clusters using singleR
singleR
singlecell
updated 3.1 years ago by
Aaron Lun
★ 28k • written 3.1 years ago by
lirongrossmann
▴ 40
0
votes
3
replies
1.6k
views
marker genes in singleR
singleR
singlecell
singlecellexperiment
updated 3.1 years ago by
Aaron Lun
★ 28k • written 3.1 years ago by
lirongrossmann
▴ 40
0
votes
1
reply
1.1k
views
Error in .updateHubDB(hub_bfc, .class, url, proxy, localHub) :
annotation
singlecell
rna-seq
updated 3.6 years ago by
shepherl
3.5k • written 3.6 years ago by
panxingxin16
• 0
50 results • Page
1 of 1
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Replies
Comment: biomaRt code/getBM function no longer importing 'coding' sequence via seqType ar
by
James W. MacDonald
63k
I should also point out that you can find this out by yourself by going to [ensembl][1], clicking on the Biomart link, and then scrolling t…
Answer: biomaRt code/getBM function no longer importing 'coding' sequence via seqType ar
by
James W. MacDonald
63k
It's my understanding that the archived Biomart data are not complete, which is likely why you cannot get sequences. But you apparently jus…
Comment: ComBat_seq function in sva package does not return a matrix
by
Federico
▴ 10
Thanks for your help, problem solved :)
Answer: ComBat_seq function in sva package does not return a matrix
by
ATpoint
★ 3.4k
That happens if your `x` is a data.frame rather than a matrix. It is listed under the Q&A section in https://github.com/zhangyuqing/ComBat-…
Answer: error running exp2grn
by
Fabricio Almeida-Silva
▴ 40
Hi, Adriana. It seems like the gene IDs you specified in `Host13tfs$Protein.ID` do not match any gene IDs in the row names of your expr…
Votes
ComBat_seq function in sva package does not return a matrix
Answer: ComBat_seq function in sva package does not return a matrix
Answer: ComBat_seq function in sva package does not return a matrix
Answer: ComBat_seq function in sva package does not return a matrix
Answer: ComBat_seq function in sva package does not return a matrix
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48k
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to
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